Figure 5.
Phloretin ameliorated the fungal activity in the murine model of oral candidiasis. Mice were sublingually infected with C. albicans suspension (1 × 107 CFU/mL) for 75 min. Topical treatments of phloretin (3.73 or 7.46 mg/mL, which were equivalent to 50× or 100× MIC) were applied twice a day starting on day one post-infection. (a) The score of lesions on the tongue tissues of mice for five days. (b) The CFU counts of C. albicans from the tongue tissues of mice with oral candidiasis. (c) The PAS staining of tongue tissues of mice. Arrows: C. albicans cells. Scale bar = 100 μm. (d) The H&E staining of tongue tissues of mice. Arrows: inflammatory cells. Scale bar = 100 μm. (e-h) The concentration of IL-6, TNF-α, IL-10, and IFN-γ. **p < 0.01 versus Sham; #p < 0.05, ##p < 0.01 versus Oral candidiasis