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. 2021 Sep 5;12(1):6057–6069. doi: 10.1080/21655979.2021.1967711

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© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — MiR-29b-3p promotes osteogenic differentiation by inhibiting NNMT.(a) The NNMT mRNA expression in BMSCs after transfection of sh-NNMT #1, sh-NNMT 2# or sh-NNMT 3#. (b) The NNMT mRNA expression in BMSCs after transfection of sh-NNMT #1 or antagomiR miR-29b-3p. (c) The expression of ALP mRNA in BMSCs after transfection of antagomiR miR-29b-3p or sh-NNMT #1. (d) The expression of RUNX2 mRNA in BMSCs after transfection of antagomiR miR-29b-3p or sh-NNMT #1. (e) The expression of RUNX2 and ALP protein in BMSCs after transfection of antagomiR miR-29b-3p or sh-NNMT #1. (f) Calcium deposition in BMSCs transfected with antagomiR miR-29b-3p or sh-NNMT #1 was detected by ARS staining

** P < 0.001 vs. blank; ## P < 0.001 vs. sh-NNMT #1.