Figure 5.

miR-136-5p directly targeted downstream XIAP. (a) Direct binding sites between miR-136-5p and WT 3ʹ-UTR of XIAP are shown. MUT 3ʹ-UTR of circ_RANBP9 was also designed. (b) Luciferase activity was tested in HEK293T cells transfected by WT XIAP or MUT XIAP recombinant plasmids and miR-136-5p mimic or mimic nc. (c) Interaction between XIAP and miR-136-5p was assessed using RNA pull-down assay. (d) XIAP expression was determined by RT-qPCR in overexpression or inhibition of miR-136-5p cells. (e) Comparison of XIAP using RT-qPCR in IOSE80 cells and GCs (KGN, COV434). (f) The expression of XIAP was measured using western blotting. **P < 0.01. ^##P < 0.01