Figure 4.

CCND1 was negatively regulated by miR-106b

(a) Schematic representation of the wild-type (WT) and mutant-type (MT) CCND1 3′-UTR luciferase reporter system, of which CCND1-WT 3′-UTR possesses a putative binding site for miR-106b-5p.B. 3′-UTR luciferase activity of WT and MT CCND1 in 293 T cells after miR-106b-5p mimic transfection. (c) RT-PCR analysis showing relative mRNA expression of CCND1 in NCI-H1299 cells with miR-106b-5p mimic transfection. (d) Expression relationship between CCND1 and miR-106b in 35 NSCLC patients.