Figure 1.

Chrysophanol suppressed cell viability while promoting cell apoptosis in glioma cells. (a) The structural formula of chrysophanol. (b) Cell viability at 48 h of U251 and SHG-44 cells was detected by 3-(4,5-Dimethyl-2-Thiazolyl)-2,5-Diphenyl Tetrazolium Bromide (MTT) assay after treatment with different concentration of chrysophanol. (c) Apoptosis rates of U251 and SHG-44 cells were assessed by Annexin V-FITC Apoptosis Detection Kit with flow cytometry after treatment with different concentration of chrysophanol. (d) Representative images of cell apoptosis in U251 and SHG-44 cells through Annexin V-FITC Apoptosis Detection Kit with flow cytometry after treatment with different concentration of chrysophanol. *p < 0.05, *** p < 0.001 vs. Control group. All experiments were repeated independently at least three times. Data were expressed as the means ± standard deviation