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. 2021 Sep 14;12(1):6855–6868. doi: 10.1080/21655979.2021.1972079

Figure 4.

Figure 4.

MitoTempo partly reversed the effect of chrysophanol on promoting reactive oxygen species (ROS) accumulation of mitochondria and inducing cell apoptosis in glioma cells. (a) Representative images of ROS detection by flow cytometry after treatment with chrysophanol and MitoTempo. (b) Flurescence intensity in U251 and SHG-44 was evaluated by flow cytometry after treatment with chrysophanol and MitoTempo. (c) Apoptosis rates of U251 and SHG-44 cells were assessed by Annexin V-FITC Apoptosis Detection Kit with flow cytometry after treatment with chrysophanol and MitoTempo. (d) Representative images of cell apoptosis in U251 and SHG-44 cells through Annexin V-FITC Apoptosis Detection Kit with flow cytometry after treatment with chrysophanol and MitoTempo. *** p < 0.001 vs. Control group; ^^^ p < 0.001 vs. Chrysophanol group; ## p < 0.01, ### p < 0.001 vs. MitoTempo group. All experiments were repeated independently at least three times. Data were expressed as the means ± standard deviation