Figure 5.

MitoTempo partly reversed the effect of chrysophanol on advancing leakage of Cytc C from mitochondria to cytosol in glioma cells. (a) Representative images of Cytosol Cyt C protein bands through western blot after treatment with chrysophanol and MitoTempo. β-actin was used as a loading control. (b) Cytosol Cyt C protein expression levels of U251 and SHG-44 cells were detected by western blot after treatment with chrysophanol and MitoTempo. β-actin was used as a loading control. *** p < 0.001 vs. Control group; ^{^^^} p < 0.001 vs. Chrysophanol group; ^### p < 0.001 vs. MitoTempo group. All experiments were repeated independently at least three times. Data were expressed as the means ± standard deviation