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. 2021 Nov 25;12(1):8994–9005. doi: 10.1080/21655979.2021.1992330

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© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — NNT-AS1 can directly bind to miR-137. A qRT-PCR analysis of nuclear and cytoplasmic RNA fraction in ccRCC cells. B AGO-2 RNA-IP assay followed qRT-PCR analysis showing binding capacity between NNT-AS1 and AGO2. C Left, the predicted binding site of WT NNT-AS1 or MUT NNT-AS1 with miR-137; right, luciferase activity was detected in HEK-293 T cells. D and E Effect of the NNT-AS1 knockdown or overexpression on miR-137 expression. F Correlation analysis between NNT-AS1 and miR-137. *P < 0.05, ** P < 0.01, ***P < 0.001