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. 2021 Aug 2;36(2):348–360. doi: 10.1038/s41375-021-01361-8

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© The Author(s), under exclusive licence to Springer Nature Limited 2021

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Fig. 7 — A PF382 and KOPTK1 cells were grown to methotrexate resistance. Parental and methotrexate resistant cells were tested with a range of methotrexate concentrations and viability evaluated at day 6 by an ATP-based assay as the percentage of viable cells relative to a DMSO control. Shown are the mean ± SD of four replicates. B On the left, bar graph showing SLC19A1 expression in methotrexate resistant PF382 and KOPTK1 cells compared to controls as mean expressions ± SD of four replicates. On the right, western blot showing level of expression of DHFR in methotrexate resistant cells compared to parental cell lines. C Methotrexate resistant PF382 and KOPTK1 cells were tested for sensitivity to RZ-2994 with a range of concentrations and viability evaluated at day 6 by an ATP-based assay as the percentage of viable cells relative to a DMSO control. Shown are the mean ± SD of four replicates.