Skip to main content
NCBI home page
NIHPA Author Manuscripts logoLink to NIHPA Author Manuscripts
. Author manuscript; available in PMC: 2022 Feb 2.
Published in final edited form as: Free Radic Biol Med. 2021 Apr 20;169:181–186. doi: 10.1016/j.freeradbiomed.2021.04.009

Suppression of mitochondrial respiration by hydrogen sulfide in hibernating 13-lined ground squirrels

Birgitte S Jensen a,b, Sibile Pardue c, Brynne Duffy b, Christopher G Kevil c, James F Staples b,1, Angela Fago a,*,1
PMCID: PMC8809085  NIHMSID: NIHMS1770835  PMID: 33887435

Abstract

Hibernating mammals may suppress their basal metabolic rate during torpor by up to 95% to reduce energy expenditure during winter, but the underlying mechanisms remain poorly understood. Here we show that hydrogen sulfide (H2S), a ubiquitous signaling molecule, is a powerful inhibitor of respiration of liver mitochondria isolated from torpid 13-lined ground squirrels, but has a weak effect on mitochondria isolated during summer and hibernation arousals, where metabolic rate is normal. Consistent with these in vitro effects, we find strong seasonal variations of in vivo levels of H2S in plasma and increases of H2S levels in the liver of squirrels during torpor compared to levels during arousal and summer. The in vivo changes of liver H2S levels correspond with low activity of the mitochondrial H2S oxidizing enzyme sulfide:quinone oxidoreductase (SQR) during torpor. Taken together, these results suggest that during torpor, H2S accumulates in the liver due to a low SQR activity and contributes to inhibition of mitochondrial respiration, while during arousals and summer these effects are reversed, H2S is degraded by active SQR and mitochondrial respiration rates increase. This study provides novel insights into mechanisms underlying mammalian hibernation, pointing to SQR as a key enzyme involved in the control of mitochondrial function.

Keywords: H2S, Hypometabolism, Ictidomys tridecemlineatus, sulfide:quinone oxidoreductase, Torpor

1. Introduction

Hibernation is a powerful way of suppressing metabolic rate which allows some mammalian species to save energy during winter [1]. Hibernating 13-lined ground squirrels (Ictidomys tridecemlineatus) spend most of the winter in torpor, a state where metabolic rate is suppressed by ~95% and body temperature (Tb) drops to ~5 °C. Torpor bouts are regularly interrupted by short arousals of ~10–12 h to interbout euthermia (IBE), during which metabolic rate and Tb rapidly return to normal levels [2]. The mechanisms underlying the dramatic metabolic suppression of torpor are complex and not fully understood [3]. It is, however, well established that mitochondrial function parallels the whole-animal metabolic variation between torpor and IBE during winter hibernation. As case in point, respiration rates under phosphorylating conditions of liver mitochondria isolated from 13-lined ground squirrels are ~70% lower in torpor than in IBE [4,5]. This suppression of mitochondrial metabolism occurs without major changes in either tissue mitochondrial density [6] or mitochondrial content of electron transport system (ETS) complexes [7]. Instead, changes in the activities of ETS complex I and II correspond with changes in the phosphorylation state of their subunits [8]. While these post-translational modifications are important in the switch of mitochondrial function between torpor and IBE during winter hibernation, it is possible that additional control mechanisms in the ETS can further contribute to suppress respiration rates.

The gasotransmitter hydrogen sulfide (H2S) has been implicated in the metabolic depression of hibernation due to its potent in vitro inhibitory effect on cytochrome c oxidase [9-11], the O2 consuming complex IV of the ETS. In support of this view, studies have reported that exogenous H2S is capable of inducing a state of suspended animation in mice [12] similar to natural hibernation, although some controversy exists [3]. Moreover, other studies by us and others have revealed significant changes in the endogenous levels and composition of sulfur metabolites in the plasma of 13-lined squirrels [13], in the blood of brown bears [14] and in the lung of Syrian hamsters [15] during torpor or hibernation. As a signaling molecule, H2S is ubiquitously produced by several enzymes [16] and it has been reported that H2S synthesis increases during torpor by increased activity of cystathionine β-synthase (CBS) and increased availability of substrates cysteine and cystathionine [3,13,15]. H2S is also continuously oxidized in the mitochondria by the membrane-bound sulfide:quinone oxidoreductase (SQR), controlling H2S homeostasis and supplying electrons to complex III of the ETS [17]. Here, we present evidence supporting that in 13-lined ground squirrels SQR activity is inhibited during torpor, which leads to accumulation of H2S with consequent inhibition of mitochondrial respiration. Taken together, these results demonstrate that H2S plays a key role in the metabolic suppression of mammalian hibernation.

2. Materials and methods

2.1. Animals

13-lined ground squirrels (Ictidomys tridecemlineatus) were trapped during late spring in Carman, MB, Canada (49°30′N, 98°01′W) with permission from Manitoba Conservation or bred in captivity at University of Western Ontario following established protocols [18]. During summer, body temperature radio telemeters (TA-ETAF10, Data Sciences International, St. Paul, MN) were implanted in the abdomen of animals [5]. In October, the animals were transferred to a hibernation chamber where the temperature was reduced by 1 °C/day until it reached 5 °C, at which point it was held constant for the duration of the hibernation season (January–March) and the photoperiod was set to 2 h light: 22 h dark [7].

Animals were euthanized and tissues sampled during three stages: 1) summer; euthermic animals, and winter hibernating, including 2) torpor; defined by a stable Tb of 5° ± 3 °C for 4–5 days, and 3) IBE; defined by a stable Tb near 37 °C for 2–3 h following a spontaneous arousal. Summer and IBE animals were euthanized by a lethal dose of Euthanyl (54 mg/100g), which does not affect mitochondrial metabolism [19]. Torpid animals were euthanized by cervical dislocation, as handling and anesthetic injection would initiate arousal.

All animal procedures were approved by the local Animal Care Committee (protocol 2012-016) of the University of Western Ontario and conformed to the guidelines of the Canadian Council on Animal Care.

2.2. Plasma and liver sampling

Blood was collected in heparin-coated vacutainers from the inferior vena cava in summer, and in winter, blood was collected following decapitation, as venous blood pressure was insufficient. Blood samples were centrifuged and 250 μL plasma was added to 800 μL conservation buffer (100 mM Tris-HCl, pH 9.5, 0.1 mM diethylenetriaminepenta-acetic acid, DTPA), where the alkaline pH converts H2S to the anionic form HS− avoiding dissipation of H2S. Likewise, ~200 mg liver was preserved in 800 μL conservation buffer. All samples were snap-frozen in liquid N2 for later measurement of bioavailable H2S. The remaining liver was stored in ice-cold homogenization buffer (250 mM sucrose, 10 mM HEPES, 1 mM EGTA, pH 7.4) and used immediately for isolation of mitochondria.

2.3. Bioavailable H2S

Bioavailable H2S was measured using a fluorescent monobromobimane assay as previously described [20], following extensively validated and refined protocols [21,22]. Sample H2S was derivatized by 30 min incubation with excess monobromobimane at 1% O2 using a hypoxic chamber in the dark at room temperature. The product of the reaction of monobromobimane with H2S, sulfide dibimane, was separated from unreacted monobromobimane by RP-HPLC using a fluorescent detector (λex: 390 nm and λem: 475 nm) and quantified after calibration with known H2S concentrations. Although monobromobimane does not significantly extrude sulfur of proteins [23], some protein-bound sulfane sulfur mobilization may occur under certain conditions [24].

2.4. Isolation of mitochondria

We used liver mitochondria, in which metabolic suppression is particularly pronounced during torpor compared with other tissues [5,25,26]. Mitochondria were isolated using differential centrifugation and purified by a Percoll gradient [27]. Purified mitochondria were suspended in 1 ml of homogenization buffer with 1% (w/v) BSA, and used immediately for measurement of mitochondrial respiration and H2S consumption.

2.5. Mitochondrial respiration

Oxygen consumption of isolated liver mitochondria was measured using an Oxygraph-2K high-resolution respirometer (Oroboros Instruments, Austria) as previously described [7]. State 2 respiration rates were determined after the addition of rotenone (2.5 μM, dissolved in 95% ethanol) and succinate (6 mM, dissolved in respiration medium) since the suppression of liver mitochondrial metabolism during torpor is greatest with succinate as oxidative substrate [4,5]. When the concentration of O2 within the respirometry chambers reached ~160 μM, various concentrations of Na2S were added (1.6–3.9 μM), to test the effect of H2S on respiration. Anaerobic Na2S stock solutions were freshly prepared (Na2S•9H2O in N2-equilibrated milli-Q water, ~8 mM).

2.6. Mitochondrial H2S consumption

An amperometric H2S-specific microsensor (customized SULF-microsensor, Unisense A/S, Aarhus, Denmark) coupled to a Unisense PA2000 amplifier was used to determine mitochondrial consumption rates of externally added H2S (as Na2S). The microsensor tip is covered by a membrane that only allows H2S to selectively pass and react with the electrolyte solution. To identify the site of H2S consumption, summer mitochondria were pre-incubated for 10 min with rotenone (2.5 μM) or antimycin A (2.5 μM) to inhibit ETS complex I and III, respectively, before H2S consumption was measured. Mitochondrial H2S consumption was also measured in sonicated mitochondria (2 × 10 s on ice).

2.7. SQR activity assay

SQR activity of previously frozen liver mitochondria from summer, torpid and IBE squirrels was obtained by following the reduction rate of decyl-ubiquinone at 275 nm upon Na2S addition [28].

2.8. Statistical analysis

Statistical analyses were performed using Prism (GraphPad Software Inc, La Jolla, CA, USA). A Shapiro-Wilk test confirmed normal distribution of data. Significant differences among hibernation states were assessed by one-way ANOVA with Tukey’s multiple comparisons test. Significant differences are indicated (*P < 0.05). Data are reported as means ± SEM, unless otherwise stated.

3. Results and discussion

We first measured bioavailable H2S in plasma and liver from winter (torpid and IBE) and summer active 13-lined squirrels to search for in vivo changes. Plasma H2S levels were significantly higher in summer than in winter hibernating squirrels, where levels did not differ significantly between torpor and IBE (Fig. 1A). H2S in plasma reflects extracellular enzymatic production by cystathionine γ-lyase (CSE) and CBS secreted by endothelial cells and liver [29] or H2S produced in the red blood cells [14,30]. Interestingly, similar plasma H2S values were detected in winter hibernating and summer brown bears using the same method [14], suggesting common seasonal patterns of sulfide transport in the circulation of hibernators.

Fig. 1.

Fig. 1.

Open in a new tab

In vivo H2S levels and H2S-induced inhibition of mitochondrial respiration. (A and B) H2S levels in plasma and liver of summer and winter hibernating squirrels (interbout euthermia [IBE] and torpor). Individual values are shown with mean ± SEM. Different letters (a–b) indicate a significant difference, P < 0.05. (C–E) Representative traces of respiration rate of liver mitochondria isolated during summer, IBE and torpor. Arrows indicate additions of 2.5 μM rotenone (R), 6 mM succinate (S) and H2S (added as Na2S). Mitochondria (10–30 μl, ~200 μg protein) were transferred to the respiration chamber containing 2 ml of mitochondrial respiration medium (0.5 mM EGTA, 3 mM MgCl2, 60 mM l-lactobionate, 20 mM taurine, 10 mM KH2PO4, 20 mM HEPES, 110 mM sucrose, 1 g/l fatty acid-free BSA, pH 7.1) at 37 °C and constant stirring (750 rpm). Electrodes were calibrated daily to 0% oxygen (using a yeast suspension) and air-saturation. Respiration rates were expressed relative to protein concentration determined using a protein assay dye (Bio-Rad). (F) Relative respiration rate (minimum rate after Na2S addition normalized to initial respiration rate) plotted as a function of added Na2S. Data are shown as mean ± SD, N = 7 (summer) and N = 2 (IBE and torpor).

In the liver of 13-lined squirrels, bioavailable H2S was significantly higher in torpor compared with IBE and summer (Fig. 1B). To determine the effect of H2S on liver mitochondria during torpor, we measured H2S-dependent respiration rates in isolated mitochondria using high-resolution respirometry. In all three states, H2S additions rapidly inhibited respiration rates (Fig. 1C-E) in a concentration-dependent fashion (Fig. 1F), but the time course and magnitude differed. In IBE and summer mitochondria, the H2S-dependent inhibition of respiration was modest and transient, and respiration rates largely recovered after few minutes (Fig. 1C and D), similar to what has been observed in human cells [31]. Conversely, in mitochondria isolated from torpid squirrels, inhibition of respiration was more pronounced and was maintained for at least 70 min at all H2S concentrations examined (Fig. 1E and F). Although these effects can in principle originate from different sensitivities of complex IV to H2S, a previous study found no difference in in vitro complex IV activity measured in liver mitochondria from 13-lined squirrels in torpor and IBE [7]. Taken together, these data suggest that the strong inhibition of complex IV by H2S observed during torpor (Fig. 1E) is achieved not by post-translational modifications of complex IV, but by changes in the mitochondrial rate of H2S oxidation, which would alter the levels of free H2S available for inhibition. Since free H2S in tissues is readily lost during isolation of mitochondria, these effects on complex IV function have remained unnoticed in previous in vitro studies.

To establish whether the H2S oxidation capacity of mitochondria was lower during torpor, we examined whether and how liver mitochondria isolated from torpid, IBE and summer squirrels differed in the oxidation rate of externally added H2S using an amperometric H2S-sensitive microsensor. We found that torpid mitochondria oxidized H2S slower than IBE and summer mitochondria (Fig. 2A) and that the rate of H2S oxidation was linearly dependent on the amount of mitochondria added (Fig. 2B and C). In addition, disruption of mitochondria membrane by sonication (Fig. 2B and C) or addition of antimycin A, an inhibitor of complex III, to intact mitochondria (Fig. 2D) abolished H2S oxidation, whereas rotenone, an inhibitor of complex I, had no effect (Fig. 2D). Taken together, these data indicate that the membrane enzyme SQR, which is positioned downstream of complex I and upstream of complex III, was responsible for H2S oxidation. To confirm these results, we measured SQR activity in liver mitochondria and found that it was significantly lower during torpor compared with summer, whereas activity during IBE was intermediate (Fig. 2E). In agreement with these results, SQR knockdown in human cancer cells increases the inhibitory effect of H2S on respiration [32]. The molecular mechanism underlying suppression of SQR activity during torpor in hibernating squirrels remains to be identified. While changes in SQR expression during IBE and torpor may be one way of changing SQR activity, the suppression of liver mitochondrial respiration happens very rapidly during entrance into torpor [33]. Moreover, the low temperatures during torpor and early arousal cause a near complete suppression of transcription and translation [34], whereby upregulation of SQR during arousal appears unlikely. Rapid and reversible posttranslational modifications may be offer a more timely and energetically favorable mechanism for changing SQR activity. Specifically, protein acetylation appears as a pervasive modification during torpor in 13-lined squirrel liver [34] and an unknown mitochondrial protein with the same molecular mass as SQR (~50 kDa) was found to be differentially acetylated between torpor and IBE in 13-lined ground squirrel liver mitochondria [8], consistent with the presence in mouse SQR of a acetylation site [35]. Future targeted transcriptomic and proteomic studies are needed to unravel SQR regulation in hibernating 13-lined squirrels. The H2S-sensitive transcription factor nuclear factor-erythroid 2-related factor 2 (Nrf2), which increases during torpor in the liver 13-lined squirrels [36], may also be involved in regulating SQR expression during hibernation and especially between summer and winter, as suggested in mice fibroblast [37]. This aspect too remains to be investigated.

Fig. 2.

Fig. 2.

Open in a new tab

Decreased mitochondrial H2S oxidation and SQR activity during torpor. (A) Representative traces of H2S consumption rates by liver mitochondria (0.5 mg protein/mL) freshly isolated from a summer active, an IBE and a torpid squirrel shown as mean of technical duplicates. Consumption rate was measured using a H2S microsensor introduced through a thin capillary into a sealed 1 ml glass chamber containing PBS buffer (1.37 mM NaCl, 28.8 μM KCl, 100 μM Na2HPO4, 17.6 μM KH2PO4 pH 7.4) at constant stirring and submerged in a water bath at 37 °C. Na2S (500 nM) was injected in the chamber via a thin injection port, followed by injections of freshly isolated liver mitochondria from torpid, IBE and summer animals, and the H2S consumption was followed for ~20 min. The microsensor was calibrated with freshly made anaerobic Na2S stock solutions. (B) H2S consumption by summer mitochondria at various amounts of mitochondria. Controls with buffer (grey) and sonicated (S) mitochondria (0.027 mg protein/mL, dashed red line) are shown. (C) H2S concentrations at 8 min in (B) plotted as a function of mitochondrial protein concentration. (D) H2S consumption rate of liver mitochondria from summer squirrels in the presence of rotenone or antimycin A relative to control. Individual values are shown with mean ± SEM. (E) SQR activity of liver mitochondria. Individual values are shown with mean ± SEM. Different letters indicate a significant difference, P < 0.05. Frozen mitochondria were carefully thawed and suspended in 20 mM Tris-HCl buffer, pH 7.8 in sealed 1-cm-quartz cuvettes containing KCN (2 mM), rotenone (40 μM) and decyl-ubiquinone (150 μM). The background rate was followed for ~5 min at 275 nm, 37 °C, before the reaction was initiated with 100 μM Na2S and followed for 5–10 min in technical triplicates. The SQR activity was calculated as the background-corrected ΔA275/time over ~2 min intervals, using an extinction coefficient of 15 L−mmol− cm−1 28 and normalized to the protein concentration, calculated from the initial A280 using an experimentally determined extinction coefficient calculated from a standard curve of known mitochondrial protein concentration. (F) Proposed H2S-dependent control of mitochondrial respiration in 13-lined ground squirrels during summer and IBE (upper panel) and torpor (lower panel). Ac: Putative acetylation, Cyt c: cytochrome c, Q: ubiquinone, IMS: intermembrane space, Sox: Oxidized sulfur, e.g. thiosulfate or sulfite.

Based on our data, we propose a model that accounts for the H2S-mediated depression of mitochondrial respiration during hibernation (Fig. 2F). During summer and IBE, H2S is degraded by active SQR and mitochondrial respiration is normal. During torpor, SQR activity is inhibited, which leads to a lower rate of H2S oxidation and to a sufficient build-up of H2S to inhibit complex IV. The consequent depression of mitochondrial respiration during torpor would then match oxygen consumption with the low oxygen supply deriving from low rates of pulmonary ventilation and heart rates [1]. In conclusion, we found a powerful inhibitory effect of H2S on mitochondrial respiration of hibernating 13-lined ground squirrels during torpor, paralleled by increased in vivo levels of bioavailable H2S in the liver. Although the proposed mechanism does not exclude a possible role of H2S-synthetising enzymes, the strongly depressed oxidation of H2S by the mitochondrial enzyme SQR provides compelling evidence that the H2S catabolic pathway is a key regulatory site for the regulation of metabolism during hibernation.

Acknowledgments

We thank Kathrine E. Mathers for squirrel illustrations. This work was supported by the Aarhus University Research Foundation (NOVA grant AUFF-E-2016- 9-37 to A.F.), by the National Institutes of Health (Institutional Development Award, IDeA grant number P20GM121307, and grant number HL149264 to C.G.K.). B.S. J. thanks the Royal Danish Academy of Sciences and Letters, and Torben and Alice Frimodts Foundation for travel grants supporting research stay at University of Western Ontario, London, Canada.

Abbreviations:

CBS

Cystathionine β-synthase

CSE

Cystathionine γ-lyase

ETS

Electron transport system

H2S

Hydrogen sulfide

IBE

Interbout euthermia

SQR

sulfide:quinone oxidoreductase

Tb

Body temperature.

Footnotes

Declaration of competing interest

The authors declare no competing interests. C.G.K. has intellectual property regarding sulfide measurement technologies and commercial interest in Innolyzer LLC.

References

  • [1].Heldmaier G, Ortmann S, Elvert R, Natural hypometabolism during hibernation and daily torpor in mammals, Respir. Physiol. Neurobiol 141 (2004) 317–329. [DOI] [PubMed] [Google Scholar]
  • [2].Staples JF, Metabolic flexibility: hibernation, torpor, and estivation, Comp. Physiol 6 (2016) 737–771. [DOI] [PubMed] [Google Scholar]
  • [3].Giroud S, Habold C, Nespolo RF, Mejías C, Terrien J, Logan SM, Henning RH, Storey KB, The torpid state: recent advances in metabolic adaptations and protective mechanisms†, Front. Physiol 11 (2021) 1–24. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • [4].Staples JF, Metabolic suppression in mammalian hibernation: the role of mitochondria, J. Exp. Biol 217 (2014) 2032–2036. [DOI] [PubMed] [Google Scholar]
  • [5].Muleme HM, Walpole AC, Staples JF, Mitochondrial metabolism in hibernation: metabolic suppression, temperature effects, and substrate preferences, Physiol. Biochem. Zool 79 (2006) 474–483. [DOI] [PubMed] [Google Scholar]
  • [6].Mathers KE, Staples JF, Saponin-permeabilization is not a viable alternative to isolated mitochondria for assessing oxidative metabolism in hibernation, Biol. Open 4 (2015) 858–864. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • [7].Mathers KE, McFarlane SV, Zhao L, Staples JF, Regulation of mitochondrial metabolism during hibernation by reversible suppression of electron transport system enzymes, J. Comp. Physiol. B 187 (2017) 227–234. [DOI] [PubMed] [Google Scholar]
  • [8].Mathers KE, Staples JF, Differential posttranslational modification of mitochondrial enzymes corresponds with metabolic suppression during hibernation, Am. J. Physiol. Integr. Comp. Physiol 317 (2019) R262–R269. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • [9].Cooper CE, Brown GC, The inhibition of mitochondrial cytochrome oxidase by the gases carbon monoxide, nitric oxide, hydrogen cyanide and hydrogen sulfide: chemical mechanism and physiological significance, J. Bioenerg. Biomembr 40 (2008) 533–539. [DOI] [PubMed] [Google Scholar]
  • [10].Nicholls P, Marshall DC, Cooper CE, Wilson MT, Sulfide inhibition of and metabolism by cytochrome c oxidase, Biochem. Soc. Trans 41 (2013) 1312–1316. [DOI] [PubMed] [Google Scholar]
  • [11].Collman JP, Ghosh S, Dey A, Decréau RA, Using a functional enzyme model to understand the chemistry behind hydrogen sulfide induced hibernation, Proc. Natl. Acad. Sci. U. S. A 106 (2009) 22090–22095. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • [12].Blackstone E, Morrison M, Roth MB, H2S induces a suspended animation–like state in mice, Science 308 (2005) 518, 518. [DOI] [PubMed] [Google Scholar]
  • [13].D’Alessandro A, Nemkov T, Bogren LK, Martin SL, Hansen KC, Comfortably numb and back: plasma metabolomics reveals biochemical adaptations in the hibernating 13-lined ground squirrel, J. Proteome Res 16 (2017) 958–969. [DOI] [PubMed] [Google Scholar]
  • [14].Revsbech IG, Shen X, Chakravarti R, Jensen FB, Thiel B, Evans AL, Kindberg J, Frøbert O, Stuehr DJ, Kevil CG, et al. , Hydrogen sulfide and nitric oxide metabolites in the blood of free-ranging brown bears and their potential roles in hibernation, Free Radic. Biol. Med 73 (2014) 349–357. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • [15].Talaei F, Bouma HR, Hylkema MN, Strijkstra AM, Boerema AS, Schmidt M, Henning RH, The role of endogenous H2S formation in reversible remodeling of lung tissue during hibernation in the Syrian hamster, J. Exp. Biol 215 (2012) 2912–2919. [DOI] [PubMed] [Google Scholar]
  • [16].Giuffré A, Vicente JB, Hydrogen sulfide biochemistry and interplay with other gaseous mediators in mammalian physiology, Oxid. Med. Cell. Longev (2018) 1–31, 2018. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • [17].Hildebrandt TM, Grieshaber MK, Three enzymatic activities catalyze the oxidation of sulfide to thiosulfate in mammalian and invertebrate mitochondria, FEBS J. 275 (2008) 3352–3361. [DOI] [PubMed] [Google Scholar]
  • [18].Vaughan DK, Gruber AR, Michalski ML, Seidling J, Schlink S, Capture, care, and captive breeding of 13-lined ground squirrels, Spermophilus trideeemlineatus, Lab Anim. (NY) 35 (2006) 33–40. [DOI] [PubMed] [Google Scholar]
  • [19].Takaki M, Nakahara H, Kawatani Y, Utsumi K, Suga H, No suppression of respiratory function of mitochondria isolated from the hearts of anesthetized rats with high-dose pentobarbital sodium, Jpn. J. Physiol 47 (1997) 87–92. [DOI] [PubMed] [Google Scholar]
  • [20].Shen X, Kolluru GK, Yuan S, Kevil CG, Measurement of H2S in vivo and in vitro by the monobromobimane method, Methods Enzymol. 554 (2015) 31–45. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • [21].Shen X, Pattillo CB, Pardue S, Bir SC, Wang R, Kevil CG, Measurement of plasma hydrogen sulfide in vivo and in vitro, Free Radic. Biol. Med 50 (2011) 1021–1031. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • [22].Shen X, Chakraborty S, Dugas TR, Kevil CG, Hydrogen sulfide measurement using sulfide dibimane: critical evaluation with electrospray ion trap mass spectrometry, Nitric Oxide 41 (2014) 97–104. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • [23].Montoya LA, Shen X, McDermott JJ, Kevil CG, Pluth MD, Mechanistic investigations reveal that dibromobimane extrudes sulfur from biological sulfhydryl sources other than hydrogen sulfide, Chem. Sci 6 (2015) 294–300. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • [24].Ditrói T, Nagy A, Martinelli D, Rosta A, Kožich V, Nagy P, Comprehensive analysis of how experimental parameters affect H2S measurements by the monobromobimane method, Free Radic. Biol. Med 136 (2019) 146–158. [DOI] [PubMed] [Google Scholar]
  • [25].Brown JCL, Staples JF, Substrate-specific changes in mitochondrial respiration in skeletal and cardiac muscle of hibernating thirteen-lined ground squirrels, J. Comp. Physiol. B 184 (2014) 401–414. [DOI] [PubMed] [Google Scholar]
  • [26].Brown JCL, Chung DJ, Belgrave KR, Staples JF, Mitochondrial metabolic suppression and reactive oxygen species production in liver and skeletal muscle of hibernating thirteen-lined ground squirrels, AJP Regul. Integr. Comp. Physiol 302 (2012) R15–R28. [DOI] [PubMed] [Google Scholar]
  • [27].Petit PX, O’Connor JE, Grunqald D, Brown SC, Analysis of the membrane potential of rat- and mouse-liver mitochondria by flow cytometry and possible applications, Eur. J. Biochem 194 (1990) 389–397. [DOI] [PubMed] [Google Scholar]
  • [28].Theissen U, Martin W, Sulfide:quinone oxidoreductase (SQR) from the lugworm Arenicola marina shows cyanide- and thioredoxin-dependent activity, FEBS J. 275 (2008) 1131–1139. [DOI] [PubMed] [Google Scholar]
  • [29].Bearden SE, Beard RS, Pfau JC, Extracellular transsulfuration generates hydrogen sulfide from homocysteine and protects endothelium from redox stress, Am. J. Physiol. Heart Circ. Physiol 299 (2010) 1568–1576. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • [30].Vitvitsky V, Yadav PK, Kurthen A, Banerjee R, Sulfide oxidation by a noncanonical pathway in red blood cells generates thiosulfate and polysulfides, J. Biol. Chem 290 (2015) 8310–8320. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • [31].Leschelle X, Goubern M, Andriamihaja M, Blottière HM, Couplan E, Gonzalez-Barroso M-D-M, Petit C, Pagniez A, Chaumontet C, Mignotte B, et al. , Adaptative metabolic response of human colonic epithelial cells to the adverse effects of the luminal compound sulfide, Biochim. Biophys. Acta 1725 (2005) 201–212. [DOI] [PubMed] [Google Scholar]
  • [32].Libiad M, Vitvitsky V, Bostelaar T, Bak DW, Lee H-JJ, Sakamoto N, Fearon E, Lyssiotis CA, Weerapana E, Banerjee R, Hydrogen sulfide perturbs mitochondrial bioenergetics and triggers metabolic reprogramming in colon cells, J. Biol. Chem 294 (2019) 12077–12090. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • [33].Chung D, Lloyd GP, Thomas RH, Guglielmo CG, Staples JF, Mitochondrial respiration and succinate dehydrogenase are suppressed early during entrance into a hibernation bout, but membrane remodeling is only transient, J. Comp. Physiol. B Biochem. Syst. Environ. Physiol 181 (2011) 699–711. [DOI] [PubMed] [Google Scholar]
  • [34].Hindle AG, Grabek KR, Elaine Epperson L, Karimpour-Fard A, Martin SL, Metabolic changes associated with the long winter fast dominate the liver proteome in 13-lined ground squirrels, Physiol. Genom 46 (2014) 348–361. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • [35].Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, et al. , Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways, Proc. Natl. Acad. Sci. U.S.A 110 (2013) 6601–6606. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • [36].Pier M, Ni Z, McMullen DC, Storey KB, Expression of Nrf2 and its downstream gene targets in hibernating 13-lined ground squirrels, Spermophilus tridecemlineatus, Mol. Cell. Biochem 312 (2008) 121–129. [DOI] [PubMed] [Google Scholar]
  • [37].Hourihan JM, Kenna JG, Hayes JD, The gasotransmitter hydrogen sulfide induces Nrf2-target genes by inactivating the keap1 ubiquitin ligase substrate adaptor through formation of a disulfide bond between Cys-226 and Cys-613, Antioxidants Redox Signal. 19 (2013) 465–481. [DOI] [PubMed] [Google Scholar]

RESOURCES