Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Creation of conditional knockdown lines for all 18 TgPDEs. (A) CRISPR-Cas9 genome editing strategy used to append an mAID-3HA, HXGPRT cassette (with 40-bp homology flanks) to the 3′ end of each TgPDE gene in the RH TIR1-3FLAG background. (B) Diagnostic PCR confirmation of successful mAID-3HA, HXGPRT integration for each TgPDE gene. Differential genomic positions of PCR1 and PCR2 are shown in panel A. WT, wild-type parental line RH TIR1-3FLAG. Tag, specific RH TgPDE-mAID-3HA clone.
