Figure 5.

Exosomal miR-125a-5p accelerates chondrocyte migration and secretion of ECM synthetic proteins. A, The typical immunofluorescence pattern of DIO (green)-labeled BMSCs-Exosabsorbed by chondrocytes was that their nuclei were stained with DAPI (blue). B, The RT-qPCR assay to determinate the mir-125a-5p expression in transfected BMSCs. C, The RT-qPCR determination on the mir-125a-5p expression in the exosomes of transfected BMSCs. D, The RT qPCR determination on the mir-125a-5p expression in chondrocytes. E and F, Western blot analysis on the E2F2 protein in exosome-treated chondrocytes. G and H, Migration analysis on chondrocytes. I, The RT-qPCR assay on the mRNA determination of MMP-13, collagen II, aggrecan and Sox9 expressions. ** P < 0.01, represents a comparison with the exoago NC or cellago NC group