Figure 1.

TSPAN1 was high expression in human breast cancer. (a). TCGA database showed the transcript per million of TSPAN1 in breast cancer tissues and normal tissues. (b). The survival analysis of patients with high or low TSPAN1 expression in tumor tissues. (c). qPCR assays showed the mRNA levels of TSPAN1 in breast cancer tissues and adjacent tissues. (d). Immunoblot assays showed the protein levels of TSPAN1 in 8 pairs of breast cancer tissues and adjacent tissues. (e). Immunoblot assays showed the protein levels of TSPAN1 in normal breast cell line MCF-10A, and four types of breast cancer cell lines, including MCF-7, T47-D, MDA-MB-231, and SK-BR-3. (f). Immunoblot showed the expression of TSPAN1 in MCF-7 and SK-BR-3 cells upon the transfection of control or TSPAN1 siRNAs. Data are shown as mean ± SEM, *p < 0.05, **p < 0.01