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. 2021 Dec 4;12(2):10935–10944. doi: 10.1080/21655979.2021.2000198

Figure 4.

Figure 4.

DLGAP1-AS1 targeted miR-299-3p/WTAP axis. (a) Cellular location analysis of DLGAP1-AS1 was detected using subcellular fractionation analysis. (b) Bioinformatic prediction (StarBase, http://starbase.sysu.edu.cn/) inspired the complementary sites of miR-299-3p with DLGAP1-AS1 wild-type 3ʹ-untranslated regions (3ʹ-UTR). (c) Luciferase reporter assays showed the luciferase activity in the co-transfection of DLGAP1-AS1 wild type or mutant with miR-299-3p mimics. (d) Bioinformatic prediction (StarBase) found that miR-299-3p shared the complementary sites with WTAP mRNA 3ʹ-UTR wild type. Mutant sequences were constructed. (e) Luciferase reporter assays demonstrated the luciferase activity in the co-transfection of WTAP mRNA wild type or mutant with miR-299-3p mimics. (f) RT-PCR demonstrated the WTAP mRNA expression in the DLGAP1-AS1 overexpression transfection or DLGAP1-AS1 silencing transfection in MCF-7/ADR cells. (g) Correlation analysis found the WTAP expression correlation with DLGAP1-AS1 expression in BC patients’ samples. *P < 0.05, **P < 0.01 vs control group