Figure 4.

M2-EVs carried MEG3 into cells. A: MEG3 expression in M2 macrophages after Rnase A or Triton X-100 treatment was detected using RT-qPCR. B-C: MEG3 expression in tissues and cells was detected using RT-qPCR. D: M2 macrophages were transfected with Cy3 (red)-labeled meg3 and co-cultured with GFP (green)-transfected YAMC. N = 6. The experiment was repeated 3 times independently. Data in panels A/C are presented as mean ± standard deviation. Data in panels A-C were analyzed using one-way ANOVA, followed by Tukey’s multiple comparisons test, **p < 0.01