Table 1.
Primer sequence for RT-qPCR
| Name | Sequence (5ʹ-3ʹ) |
|---|---|
| MEG3 | F: ATGAGAGAGAGAACAGCGAGAA |
| R: GTCTATGGACTGAAGATGTGAC | |
| miR-20b-5p | F: GCCGAGCAAAGTGCTCATAGT |
| R: CTCAACTGGTGTCGTGGA | |
| CREB1 | F: ATGCCAGCAGCTCATGCAACAT |
| R: GATTTGTGGCAGTAAAGGTCC | |
| CCL22 | F: ATGGCTACCCTGCGTGTCCCA |
| R: CTAGGACAGTTTATGGAGTAGC | |
| PPARγ | F: ATGGTTGACACAGAGATGCC |
| R: TCCTTGTAGATCTCCTGGAGCA | |
| Bcl-2 | F: ATGGCGCAAGCCGGGAGAAC |
| R: TCACTTGTGGCCCAGGTATGCA | |
| Bax | F: TGAACAGATCATGAAGACAGG |
| R: TCAGCCCATCTTCTTCCAGATG | |
| U6 | F: GCTCGCTTCGGCAGCACATATA |
| R: GGAACGCTTCACGAATTTGCG | |
| GAPDH | F: ATGCTGCCCTTACCCCGGGGT |
| R: TTACTCCTTGGAGGCCATGTAG |
Note: RT-qPCR: reverse transcription quantitative polymerase chain reaction; MEG3: maternally expressed 3; miR-20b-5p: micro-20b-5p; CREB1: cAMP responsive element binding protein 1; CCL22: chemokine (C-C motif) ligand 22; PPARγ: peroxisome proliferator activated receptor gamma; Bcl-2: B-cell lymphoma protein 2; Bax: BCL2-associated X protein; GAPDH: glyceraldehyde-3-phosphate dehydrogenase