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. 2021 Dec 7;12(2):10194–10202. doi: 10.1080/21655979.2021.2002018

Figure 4.

Figure 4.

CENPU promoted proliferation and glycolysis of gastric cancer cells via HMGB2. (a) Left, representative blots of lysate of normal tissue adjacent to the tumor and gastric cancer tissue from gastric cancer patient. n = 40. Right, correlation analysis of the protein expression levels of CENPU and HMGB2. (b) Upper, representative blots of WB analysis of HMGB2 protein expression level after transfections with si-CENPU and pcDNA-CENPU. Lower, the relative protein expression level of HMGB2 normalized to GAPDH. ***p < 0.001 vs si-NC, ##p < 0.01 vs pcDNA. Data are mean ± SEM of at least three independent experiments. (c) MTT assay was used to assess the cell proliferation after transfection with si-CENPU and pcDNA-HMGB2 in AGS cells. **p < 0.01 vs si-NC, ##p < 0.01 vs si-CENPU. Data are mean ± SEM of at least three independent experiments. (d) Left, representative images of cell colony formation after transfections with si-CENPU and pcDNA-HMGB2 in AGS cells. Right, the average number of colonies in each dish of at least three independent experiments after transfection with si-CENPU and pcDNA-CENPU in AGS cells. **p < 0.01 vs si-NC, ##p < 0.01 vs si-CENPU. (e) Glucose consumption was detected by glucose assay kit in AGS cells. ***p < 0.001 vs si-NC, ##p < 0.01 vs si-CENPU. Data are mean ± SEM of at least three independent experiments. (f) Lactate production was assessed by lactate assay kit in AGS cells. **p < 0.01 vs si-NC, ##p < 0.01 vs si-CENPU. Data are mean ± SEM of at least three independent experiments. (g) Total ATP content was measured by ATP assay kit. **p < 0.01 vs si-NC, ##p < 0.01 vs si-CENPU. Data are mean ± SEM of at least three independent experiments. (h) Left, representative blots of WB analysis of LDHA, GLUT1 and HK2 protein expression levels after transfections with si-CENPU and pcDNA-HMGB2 in AGS cells. Right, the relative protein expression levels of LDHA, GLUT1 and HK2 normalized to GAPDH. **p < 0.01 vs si-NC, ##p < 0.01 vs si-CENPU. Data are mean ± SEM of at least three independent experiments