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. Author manuscript; available in PMC: 2022 May 29.
Published in final edited form as: Oncogene. 2021 Nov 29;41(6):757–769. doi: 10.1038/s41388-021-02131-7

Figure 3. Tenascin C is expressed in tumor-induced osteoblasts in human bone metastasis specimens and Tenascin C neutralizing antibody reduces EC-OSB-CM-mediated migratory activity in PCa cells.

Figure 3.

(A) IHC of TNC in osteogenic tumors. TNC is found in EC-OSB hybrid cells rimming tumor-induced bone in MDA-PCa-118b PDX (left) and human PCa bone metastasis (right). B, bone; T, tumor. Magnification X200. Effects of TNC neutralizing antibody BC-24 on (B) migratory activity and (C) Anchorage-independent growth of EC-OSB-CM on C4-2B4 and PC3-mm2 cells. EC-OSB-CM were pre-incubated with anti-TNC mAb BC-24 (200 ng/ml) or control anti-AE1/AE3 mAb (200 ng/mL) overnight at 4°C prior to use in migration or anchorage-independent growth assays. P values were by Student’s t-test. NS, not significant. (D) Graphical summary. One of the factors secreted by EC-OSB hybrid cells is TNC, which promotes PCa migration, invasion and anchorage-independent growth.