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. Author manuscript; available in PMC: 2022 Mar 24.
Published in final edited form as: Science. 2021 Aug 26;373(6562):1532–1536. doi: 10.1126/science.abf7461

Fig. 3. TIL is a plant lipocalin that interacts with retinal.

Fig. 3.

(A) Predicted protein structure of Arabidopsis TIL and Human RBP4 using the protein homology/analogy recognition engine V 2.0 (PHYRE2) (19). (B) The TIL expression pattern in a representative pTIL:LUC root. The asterisk indicates the spatial region analyzed in panel D. (C) A 3D surface plot showing the spatial profile of the pTIL:LUC root shown in panel B. (D) The luminescence intensity over time in the region of root highlighted by the asterisk in panel B. (E) Representative images of E. Coli cells transformed with Arabidopsis genes. (F) Fluorescence intensity of MCA-treated E. Coli transformed with genes from Arabidopsis. The control cells were not transformed. (G) Representative images of E. Coli cells expressing TIL that were pre-treated with DMSO, β-cyclocitral (Bcc), anchorene, or retinal. (H) Fluorescence intensity of MCA treated E. Coli transformed with TIL and pre-treated with DMSO, β-cyclocitral (Bcc), anchorene, or retinal.