Figure 1.

Mammalian Centrosome Architecture. The Centrosome consists of two orthogonal centrioles, embedded in the pericentriolar material (PCM) and is surrounded by small electron-dense granules (i.e., centriolar satellites). Centriolar satellites and PCM proteins are recruited towards the centrosome for centriole and microtubule assembly. Spindle microtubules are nucleated from γ-tubulin ring complexes (γ-TuRCs). The mother centriole is characterized by its sub-distal and distal appendages, whereas the daughter centriole contains a characteristic cartwheel structure. The centriolar wall consists of microtubule triplets of A-, B, and C-tubules.