Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Oct 19;41(7):943–959. doi: 10.1038/s41388-021-02060-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — A Immunofluorescence detection to visualize intracellular localization of AR (green) in LNCaP cells by high-resolution confocal scanning fluorescence microscopy. Nuclei are stained by DAPI (blue). Scale bar indicates 2 µm. B, C Intracellular detection of AKT (B) and p-AKT (C) by superresolution confocal scanning fluorescence microscopy (red). Wheat germ agglutinin (WGA) was used as membrane marker (green). Scale bar indicates 2 µm (n = 3). D Quantitative proximity-ligation assays (PLA) were performed to analyze native intracellular interaction of endogenous AR with endogenous AKT in the presence of SAL and AKTi. Cells were treated for 72 h. Shown are representative pictures. Scale bar indicates 10 µm. E The number of PLA signals per cell was counted by Fiji software. PLA signals were calculated from 80 cells derived from three independent experiments. Bar graphs are shown as mean ± SEM, ***p ≤ 0.001, n.s. not significant.

Fig. 4 — A Immunofluorescence detection to visualize intracellular localization of AR (green) in LNCaP cells by high-resolution confocal scanning fluorescence microscopy. Nuclei are stained by DAPI (blue). Scale bar indicates 2 µm. B, C Intracellular detection of AKT (B) and p-AKT (C) by superresolution confocal scanning fluorescence microscopy (red). Wheat germ agglutinin (WGA) was used as membrane marker (green). Scale bar indicates 2 µm (n = 3). D Quantitative proximity-ligation assays (PLA) were performed to analyze native intracellular interaction of endogenous AR with endogenous AKT in the presence of SAL and AKTi. Cells were treated for 72 h. Shown are representative pictures. Scale bar indicates 10 µm. E The number of PLA signals per cell was counted by Fiji software. PLA signals were calculated from 80 cells derived from three independent experiments. Bar graphs are shown as mean ± SEM, ***p ≤ 0.001, n.s. not significant.