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. 2022 Jan 19;27(3):629. doi: 10.3390/molecules27030629

Figure 3.

Figure 3

Increase in glucose uptake in differentiated myoblasts by AF. C2C12 cells were exposed to 2% horse serum for differentiation. After 6 days, cells were treated with AF (20 and 50 μg/mL) or rosiglitazone 10 μM (ROS 10). Glucose uptake was assessed by measuring 2-NBDG (50 μg/mL; ex/em ~475/550 nm) and Hoechst33342 (20 μg/mL; ex/em ~350/461 nm) uptake. (A) Representative images of cells untreated and treated with samples were acquired with 2-NBDG (in green), and Hoechst (in blue). Scale bar: 200 µm. (B) Relative intensity representing mean ± S.D. of triplicate in two independent experiments. (C) 2-NBDG uptake in ng/mL representing mean ± SEM. of triplicate in two independent experiments. # p < 0.05, compared with the DMSO treated group. * p < 0.05 compared with the 2-NBDG treated group.