Table 1.
Primer sequences of all tested Xenopus laevis genes and conditions for quantitative polymerase chain reaction (qPCR).
| Gene | Primer Sequences (5′–3′) | Annealing Temperature (°C) | GeneBank ID |
|---|---|---|---|
| rpl8 | F: CCGTGGTGTGGCTATGAATC | 58 | NM_001086996.1 |
| R: TACGACGAGCAGCAATAAGAC | |||
| klf9 | F: GTGGCCACTTGATTTCCCCT | 64 | NM_001085597.1 |
| R: AAAGACACAAAACAGCGGCG | |||
| thibz | F: CCACCTCCACAGAATCAGCAG | 62 | NM_001085805.1 |
| R: AGAAGTGTTCCGACAGCCAAG | |||
| thrb | F: GAGATGGCAGTGACAAGG | 58 | NM_001087781.1 |
| R: CAAGGCGACTTCGGTATC | |||
| st3 | F: CCTCTGTCATACACTTACCTT | 62 | NM_001086342.1 |
| R: TGAACCGTGAGCATTGAG | |||
| dio3 | F: GATGCTGTGGCTGCTGGAT | 62 | NM_001087863.1 |
| R: ATTCGGTTGGAGTCGGACAC | |||
| mmp13 | F: CCTTGTCAGTGCTTGTCCTATC | 62 | NM_001100931.1 |
| R: TCCTGGTGTCAGTTCAGAGTC |
F: forward; R: reverse; rpl8: ribosomal protein L8; klf9: Kruppel-like factor 9; thibz: thyroid hormone induced bZip protein; thrb: thyroid hormone receptor beta; st3: stromelysin-3; dio3: type 3 deiodinase; mmp13: matrix metallopeptidase 13.