Table 1.
REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Chemicals | ||
Clear-sol II | Nacalai Tesque | Cat# 09136-83 |
[8-14C]-Uric acid (53 mCi/mmol) | American Radiolabeled Chemicals | Cat# ARC0513 |
Dimethyl Sulfoxide | Nacalai Tesque | Cat# 13445-74; CAS: 67-68-5 |
Ethanol | FUJIFILM Wako Pure Chemical | 057-00451; CAS: 64-17-5 |
Methanol | FUJIFILM Wako Pure Chemical | 137-01823; CAS: 67-56-1 |
n-Hexane | FUJIFILM Wako Pure Chemical | 085-00416; CAS: 110-54-3 |
Ethyl acetate | FUJIFILM Wako Pure Chemical | 051-00356; CAS: 141-78-6 |
n-Buthanol | FUJIFILM Wako Pure Chemical | 026-03326; CAS: 71-36-3 |
Polyethelenimine “MAX” | Polysciences | Cat# 24765; CAS: 49553-93-7 |
2′-Hydroxychalcone | Tokyo Chemical Industry | Cat# H0385; CAS: 1214-47-7; Purity: >98% |
3-Hydroxyflavone | Tokyo Chemical Industry | Cat# H0379; CAS: 577-85-5; Purity: ≥98% |
4-Hydroxychalcone | Tokyo Chemical Industry | Cat# H0955; CAS: 20426-12-4; Purity: >96% |
4′-Hydroxychalcone | Tokyo Chemical Industry | Cat# H0945; CAS: 2657-25-2; Purity: >95% |
Aesculetin | FUJIFILM Wako Pure Chemical | Cat# A15393; CAS: 305-01-1; Purity: N/A |
Apigenin | FUJIFILM Wako Pure Chemical | Cat# 016-18911; CAS: 520-36-5; Purity: ≥95% |
Cardamonin | R&D systems | Cat# 2509/10; CAS: 19309-14-9; Purity: ≥98% |
Daidzein | FUJIFILM Wako Pure Chemical | Cat# 043-28071; CAS: 486-66-8; Purity: ≥98% |
Dihydromyricetin | EXTRASYNTHESE | Cat# 1351-10 mg; CAS: 27200-12-0; Purity: ≥95% |
Fisetin | LKT Labs | Cat# F3473; CAS: 528-48-3; Purity: ≥97% |
Galangin | ChromaDex | Cat# ASB-00007030-010; CAS: 548-83-4; Purity: N/A |
Genistein | FUJIFILM Wako Pure Chemical | Cat# 073-05531; CAS: 446-72-0; Purity: ≥98% |
Gossypetin | ChromaDex | Cat# ASB-00007390-010; CAS: 489-35-0; Purity: N/A |
Isoliquiritigenin | Tokyo Chemical Industry | Cat# I0822; CAS: 961-29-5; Purity: ≥97% |
Kaempferol | FUJIFILM Wako Pure Chemical | Cat# 110-00451; CAS: 520-18-3; Purity: ≥95% |
Luteolin | Cayman Chemical | Cat# 10004161; CAS: 491-70-3; Purity: ≥98% |
Morin | Combi-Blocks | Cat# QC-0527; CAS: 480-16-0; Purity: ≥98% |
Myricetin | FUJIFILM Wako Pure Chemical | Cat# 137-16791; CAS: 529-44-2; Purity: ≥98% |
Naringenin chalcone | ChromaDex | Cat# ASB-00014207-005; CAS: 73692-50-9; Purity: N/A |
Phloretin | FUJIFILM Wako Pure Chemical | Cat# 160-17781; CAS: 60-82-2; Purity: ≥98% |
Quercetagetin | ChromaDex | Cat# ASB-00017020-005; CAS: 90-18-6; Purity: N/A |
Quercetin | ChromaDex | Cat# ASB-00017030-010; CAS: 117-39-5: Purity: ≥97% |
Taxifolin | EXTRASYNTHESE | Cat# 1036; CAS: 17654-26-1; Purity: N/A |
Xanthohumol | TOKIWA PHYTOCHEMICAL | Cat# P2217; CAS: 569-83-5; Purity: ≥98% |
Critical Commercial Assays | ||
Pierce BCA Protein Assay Reagent A, B | Thermo Fisher Scientific | Cat# 23223, Cat# 23224 |
PureLink HiPure Plasmid Filter Midiprep Kit | Thermo Fisher Scientific | Cat# K210015 |
Recombinant DNA | ||
The complete human URAT1 cDNA in pEGFP-C1 | Saito et al. 2020 [14] | NCBI Reference Sequence: NM_144585.3 |
Experimental Models: Cell Lines | ||
293A | Invitrogen | R70507 |
N/A, not available.