Figure 6.

The gene expression profile of quadriceps muscle is largely unaffected by NR.

Expression of (A) Nmrk2, (B) Art3, (C) Myh8, and (D) Mymk transcripts in counts per million (CPM) from the full mRNA sequencing of quadriceps muscle.

(E) Expression of Nmrk1 transcript in iWAT from qRT-PCR analysis.

(F–I) Expression of Nampt, Cd38, Sirt3, and Sirt5 transcripts in counts per million (CPM) from the full mRNA sequencing of quadriceps muscle.

(J) Top 10 most significantly changed gene ontology terms associated with genes affected by voluntary wheel running. The size of the point indicates the number of genes in the term. The color indicates whether genes in the term tend to be up-regulated or down-regulated. Note that the x axis is on a logarithmic scale.

All data except J are presented as mean with overlain data points. The statistical analysis used for (A), (B), (C), (D), (F), (G), (H), and (I) can be found in the Method details under mRNA sequencing. Two-way ANOVA was performed for (E). Statistical significance was indicated in the following manner: Effects of intravenous NR: p < 0.05 = †, p < 0.001 = †††. Effects of VWR: p < 0.05 = § p < 0.01 = §§§. The symbols indicate the main effects. See also Figure S5.