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. 2022 Feb 15;11:e70271. doi: 10.7554/eLife.70271

Figure 3. Effects of adolescent social isolation on the novelty-suppressed feeding (NSF) assay in males and females.

(A) Experimental paradigm for the NSF assay performed in the morning following an overnight fast, in adult mice socially isolated at 5 weeks of age. (B) Latency to eat in home (H) and novel (N) tests in males (blue, n = 11, Wilcoxon test: W = 66; p = 0.001) and in females (green, n = 8, Wilcoxon test: W = 36; p = 0.0078). (C) Food intake in home (H) and novel (N) tests in males (blue, n = 11, paired t-test: t = 2.193; df = 10; p = 0.0531) and in females (green, n = 8, paired t-test: t = 6.347; df = 7; p = 0.0004). **p < 0.01, ***p < 0.001 between home and novel tests. SI, social isolation. See Figure 3—source data 1.

Figure 3—source data 1. Effects of long social isolation starting at adolescence on the standard novelty-suppressed feeding (NSF) assay.
elife-70271-fig3-data1.xlsx (628.3KB, xlsx)
Figure 3—source data 2. Parsing the effects of length vs. timing of social isolation on the standard novelty-suppressed feeding (NSF) assay in females.
elife-70271-fig3-data2.xlsx (628.6KB, xlsx)

Figure 3.

Figure 3—figure supplement 1. Parsing the effects of length vs. timing of social isolation on the standard novelty suppressed feeding (NSF) assay in females.

Figure 3—figure supplement 1.

(A) Experimental paradigm for the manual novelty-suppressed feeding (NSF) assay in adolescent and adult females. (B) Latency to eat in home (H) and novel (N) tests in adult females socially isolated from 8 weeks of age for 7 weeks (red, n = 9, Wilcoxon test: W = 43; p = 0.0078) and in 7-week-old female mice socially isolated at 5 weeks of age (green, n = 13, Wilcoxon test: W = 85; p = 0.0012). (C) Food intake in home (H) and novel (N) tests in adult females socially isolated at 8 weeks of age for 7 weeks (red, n = 9, paired t-test: t = 2.211; df = 8; p = 0.058) and in 7-week-old female mice socially isolated at 5 weeks of age (green, n = 13, Wilcoxon test: W = 18; p = 0.2656). **p < 0.01. SI, social isolation; n.s., not significant. See Figure 3—source data 2.