The response of mouse B cells to re-oxygenation in vivo. a, Spleen confocal images from immunised mice (Figure 3a), MZ B cells (magenta, B220+CD23−), FO B cells (yellow, B220+CD23+) and MZ metallophilic macrophages (blue, CD169+). B cell area, circles represent individual follicles from one spleen per condition, mean ± s.e.m. b, Experiment outline and absolute spleen B cells, (two-way ANOVA with Tukey's multiple comparisons test). Gating in methods. Mean ± s.e.m, data pooled from two experiments. *P < 0.05,**P < 0.01,***P < 0.001,**** P < 0.0001.