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Phenotypes of NESCs at different differentiation stages in vitro. (a) Quantification of living cells of cryopreserved NESCs. (b) Quantification of Sox2 positive cells at three different stages of differentiation in vitro, 0 DIV, 6 DIV and 12DIV (mean ± SEM, one-way ANOVA, p < 0·05 n = 3 batches of cells per group). (c) Differentiated NESCs (12 days in vitro) stained for Sox2 (red), beta III tubulin (Tuj1, green) and DAPI (blue). Scale bar= 30 µm.
