Extended Data Figure 8. Modulation of mNAcSh or POMC-containing neurons during sucrose consumption.

a. Schematic of hM3D(Gi) (left) and hM3D(Gq) (right) DREADD experiments. b. Fluorescent micrograph of mCherry-tagged enkephalin cells in mNAcSh for hM3D(Gi) (left) and hM3D(Gq) (right) experiments (scale bar = 200μm). c. CNO injections suppressed hunger enhanced intake in Cre+ mice, but had no effect in Cre− mice (n = 15 Cre−, 12 Cre+). d. CNO injections increased intake above the already elevated food deprived intake in Penk-Cre+ mice, but had no effect in Penk-Cre− mice (n = 7 Cre−, 10 Cre+). e. Systemic CNO administration (3mg/kg, i.p.) suppressed the already low ad libitum intake in Penk-Cre+ mice, but did not reduce intake in Penk-Cre− mice. f. Systemic CNO administration (3mg/kg, i.p.) had no effect on ad libitum intake in Penk-Cre− or Penk-Cre+ mice). g. Gq or Gi DREADD injections into arcuate nucleus of POMC-Cre mice. h. Micropictograph of mCherry-tagged, DREADD-expressing cells in arcuate nucleus (scale bar = 200 μm). i-k. Neither Gi nor Gq stimulation had an effect on ad libitum or food deprived intake in Cre− or Cre+ mice (n = 7 Cre−, 7 Gq, 9 Gi). All error bars represent ± SEM and n = biologically independent mice. Post hoc p-values are derived from Two-way ANOVA with Sidak multiple comparisons (c, d, e, f, i, j, k).