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. 2022 Feb 5;25(3):103867. doi: 10.1016/j.isci.2022.103867

Figure 5.

Figure 5

Specific Shp2 knockout in endothelium suppresses M2 activation of macrophages in injured lungs

(A) Immunofluorescence staining of irradiated lung sections with IB4 (endothelial marker, red) and F4/80 (macrophage marker, green), F4/80 positive cells that observed nearly to (≤5 μm) IB4 positive cells were considered to be accumulated. Nuclei were counterstained with DAPI (blue). Scale bar, 20 μm.

(B) qRT-PCR analysis of relative Arg1, Fizz1, and Ym1 mRNA levels in lung tissues from irradiated control and Shp2 iECKO mice.

(C) Immunofluorescence staining of Arg1 (red) and CD68 (green) in lung sections from control and Shp2 iECKO mice 6 months after irradiation. Nuclei were counterstained with DAPI (blue). Scale bar, 20 μm.

(D) qRT-PCR analysis of relative Arg1, Fizz1, and Ym1 mRNA levels in AMs from the BALF of irradiated control and Shp2 iECKO mice.

(E and F) qRT-PCR analysis of relative HES1, HEY1, Notch1, Notch2, and Notch3 mRNA levels in AMs from the BALF of irradiated control and Shp2 iECKO mice.

Data were shown as the mean ± SEM of three independent experiments. p-value was calculated using one-way or two-way ANOVA. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. See also Figure S6.