Table 1.
Pathogen | Primer | Direction | Amplicon (bp) | Targeted gene | Sequence |
---|---|---|---|---|---|
Rickettsia spp. | 107F | Forward | 209–212 | Rickettsia ompA region | 5′-GCT TTA TTC ACC ACC TCA AC-3′ |
299R | Reverse | 5′-TRA TCA CCA CCG TAA GTA AAT-3′ | |||
Ehrlichia spp. | DSB-321 | Forward | 378 | Ehrlichia dsb gene | 5′-TTG CAA AAT GAT GTC TGA AGA TAT GAA ACA-3′ |
DSB-671 | Reverse | 5′-GCT GCT CCA CCA ATA AAT GTA TCY CCT A-3′ |