a-c, TANGO screens against a panel of 320 GPCRs for the indicated σ2 ligand. a, Z4446724338, b, Z1665845742, c, Z4857158944. d, GloSensor μOR-mediated cAMP inhibition (Gi activation) by DAMGO, Z4446724338, Z1665845742, and Z4857158944. e-f, Follow-up does-response curves for pain-related receptors that showed activation in a-c. e, Z4446724338 and Z1665845742 against 5HT1A. f, Z4857158944 against κOR. Data shown are means ± SEM.