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. 2022 Jan 20;10(2):217. doi: 10.3390/biomedicines10020217

Figure 2.

Figure 2

Assessment of oxPL/apoB plasma levels in healthy donors and CVD patients and regulation of NET formation by oxLDL in primed neutrophils. (A) Boxplots illustrate the plasma levels of oxPL/apoB as determined by ELISA in healthy donors and patients with PAD or AAA. (B) Plasma levels of oxPL/apoB are depicted for AAA patients with citH3 concentrations above or below (≤) the median. Statistical significance was determined by Mann–Whitney U test. (C) NET formation of primed neutrophils is enhanced by oxLDL but not by natLDL or HDL. Neutrophils were isolated from the peripheral blood of healthy donors and were left untreated or primed for 30 min with 5 µM A23187. Neutrophils were then washed and subsequently exposed to 100 µg/mL oxLDL, natLDL or HDL (or again to 5 µM A23187) as indicated. DNA release (mean ± SEM) was quantitated after 90 min in relative fluorescence units (RFU). Statistical analysis is based on paired t-test (n = 8 assays).