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. 2022 Feb 17;11(4):712. doi: 10.3390/cells11040712

Figure 1.

Figure 1

Activation of p53 in RLDC-treated BUMPT cells and its inhibition by pifithrin-α. BUMPT cells were incubated with 2 μM cisplatin for 7 h daily for 4 days in the absence or presence of 20 μM pifithrin-α. (A) p53 immunofluorescence and DAPI staining of nuclei. Scale bar = 0.2 mm. (B) Immunoblots of p53, phospho-p53 (Ser 15), and p21 with GAPDH as internal loading marker. (C–E) Densitometric analysis of p53, p-p53 (Ser15) and p21 normalized to GAPDH. Data are expressed as means ± SD (n = 4). * p < 0.05, significantly different from the control group. # p < 0.05, significantly different from the cisplatin only group.