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. 2022 Feb 20;11(4):738. doi: 10.3390/cells11040738

Figure 2.

Figure 2

Quantification of the BLG-specific IgE, histamine, and mast cell protease-1 (MCPT-1) using ELISA. The blood samples collected from the sham and BLG-sensitized mice at weeks 0, 5, and 7 were used to detect the plasma levels of BLG-specific IgE (A). The values indicate spectrophotometric readouts at 450 nm after subtracting the reference readouts at 550 nm and are expressed as optical density (OD). Histamine (B) and MCPT-1 (C) were also quantified from the plasma and serum samples of the terminal blood, respectively, using commercial ELISA kits with respective standards. Each bar indicates the group average ± SEM (n = 10 per group). Red open circles and blue diamonds represent individual values for the sham and BLG mice, respectively. The numbers between two bars indicate the p-values. Statistical significance (p < 0.05) was determined with multiple uncorrected t-tests (BLG-specific IgE) or unpaired Student’s t-tests (histamine and MCPT-1).