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SDS-PAGE profile of HPC following pH shifting alone (a) and HPC following treatment with the combination of US with pH shifting (b). Samples were prepared with (+DTT) or without (−DTT). Std: molecular weight marker (kDa). HPC constituents: A/B (48 kDa) for Edestin; A, the acidic subunit (34 kDa), B, the basic subunit (20 and 188 kDa) for Edestin; 7S (46 kDa) for 7S globulin.
