Table 3.
Optimized hydrolysis conditions and verification model for all responses of jellyfish hydrolysate using alcalase (JFAH), flavourzyme (JFFH), and papain (JFPH) hydrolysis.
| Enzyme | Value | Factors | Responses | ||||||
|---|---|---|---|---|---|---|---|---|---|
| X1 (min) | X2 (%) | %DH | %Yield | DPPH (IC50) (mg/mL) |
ABTS (IC50) (mg/mL) |
FRAP (IC50) (mmol FeSO4/g) |
Tyrosinase Inhibitory (IC50) (mg/mL) | ||
| JFAH | Predicated value | 27.7 | 2.97 | 2.30 | 1.61 | 0.14 | 14.1 | ||
| Experimental value | 360 | 5 | 28.2 a ± 1.1 | 3.01 a ± 0.04 | 2.5 c ± 0.1 | 1.81 a ± 0.02 | 0.13 a ± 0.05 | 14.9 b ± 0.0 | |
| Composite desirability | 0.96 | ||||||||
| JFFH | Predicated value | 45.6 | 5.32 | 0.81 | 5.40 | 0.17 | 19.0 | ||
| Experimental value | 345 | 5 | 47.3 c ± 0.3 | 6.40 b ± 0.03 | 0.73 a ± 0.14 | 2.58 b ± 0.19 | 0.23 b ± 0.04 | 14.1 ab ± 0.1 | |
| Composite desirability | 0.96 | ||||||||
| JFPH | Predicated value | 40.7 | 7.45 | 0.95 | 4.30 | 0.23 | 23.5 | ||
| Experimental value | 360 | 5 | 41.8 b ± 0.5 | 7.21 bc ± 1.05 | 0.98 b ± 0.04 | 4.50 c ± 0.08 | 0.27 c ± 0.02 | 24.5 c ± 0.0 | |
| Composite desirability | 0.95 | ||||||||
Note: Mean ± SD, X1: hydrolysis time (min), X2: enzyme concentration (%); different superscripts in the same experimental values of response show significant differences (p ≤ 0.05).