Table 3.
Relative organ weight and hematological parameters of Balb/c mice in toxicological (G1 and G3) and chemically-induced skin carcinogenesis (G2 and G4) experiments at two endpoints (24th and 27th experimental week).
| Group | G1 | G2 | G3 | G4 | |||||
|---|---|---|---|---|---|---|---|---|---|
| Experimental Day | 21-Day | 42-Day | 21-Day | 42-Day | 21-Day | 42-Day | 21-Day | 42-Day | |
| Relative organ weight | Liver | 5.0 ± 0.4 | 4.8 ± 0.2 | 5.6 ± 0.4 | 4.9 ± 0.5 | 4.9 ± 0.4 | 4.6 ± 0.2 | 5.4 ± 0.5 | 5.2 ± 0.3 |
| Kidneys | 1.9 ± 0.1 | 1.9 ± 0.1 | 2.0 ± 0.1 | 1.87 ± 0.08 | 1.9 ± 0.1 | 1.9 ± 0.1 | 1.9 ± 0.2 | 1.9 ± 0.1 | |
| Testis | 0.72 ± 0.07 | 0.6 ± 0.2 | 0.7 ± 0.2 | 0.69 ± 0.07 | 0.74 ± 0.08 | 0.72 ± 0.02 | 0.7 ± 0.1 | 0.7 ± 0.1 | |
| Spleen | 0.47 ± 0.04 | 0.36 ± 0.09 | 0.6 ± 0.4 | 0.4 ± 0.1 | 0.45 ± 0.07 | 0.38 ± 0.04 | 0.6 ± 0.2 | 0.5 ± 0.2 | |
| Hematological parameters | WBC (×103 μL) | 6.1 ± 0.4 | 6.6 ± 1.6 | 6.5 ± 2.4 | 7.8 ± 0.9 | 3.8 ± 0.5 * | 7.9 ± 2.7 | 5.5 ± 1.1 | 8.0 ± 1.4 |
| RBC (×106 μL) | 11.0 ± 0.2 | 11.1 ± 0.3 | 10.9 ± 0.6 | 11.0 ± 0.6 | 11.2 ± 0.4 | 11.3 ± 0.5 | 10.7 ± 0.8 | 10.7 ± 1.1 | |
| HGB (g/dL) | 15.0 ± 0.4 | 15.2 ± 0.5 | 14.8 ± 0.7 | 15.4 ± 0.7 | 15.2 ± 0.7 | 15.2 ± 0.8 | 14.5 ± 0.8 | 15. ± 0.8 | |
| HCT (%) | 53.4 ± 1.1 | 54.3 ± 1.7 | 53.3 ± 2.3 | 54.5 ± 2.7 | 54.6 ± 1.6 | 56.1 ± 2.7 | 52.3 ± 2.6 | 53.3 ± 5.7 | |
| MCV (fL) | 48.8 ± 1.2 | 48.9 ± 0.6 | 48.6 ± 1.1 | 49.6 ± 0.2 | 48.9 ± 0.6 | 49.6 ± 0.3 | 49.1 ± 1.5 | 49.7 ± 1.1 | |
| MCH (pg) | 13.7 ± 0.2 | 13.7 ± 0.4 | 13.3 ± 0.9 | 13.7 ± 0.2 | 13.6 ± 0.2 | 13.4 ± 0.1 | 13.6 ± 0.3 | 13.4 ± 0.6 | |
| MCHC (g/dL) | 28.2 ± 1.0 | 27.9 ± 0.6 | 27.3 ± 1.7 | 27.6 ± 0.4 | 27.9 ± 0.6 | 27.2 ± 0.1 | 27.7 ± 0.6 | 27.0 ± 0.7 | |
| PLT (×103μL) | 1686 ± 92 | 1726 ± 155 | 1620 ± 304 | 1662 ± 178 | 1842 ± 272 | 1581 ± 27 | 1750 ± 308 | 1501 ± 126 | |
Results expressed as mean ± standard deviation (n = 7 at each endpoint, G1 and G3; n = 10 at each endpoint, G2 and G4). Animal: male Balb/c mice (8-week-old at the beginning of the experiment). Statistical analysis: ANOVA followed by Tukey’s test (* p < 0.05). Challenge: DMBA (7,12-dimethylbenzanthracene, single dose at S-1, 60 μg in 200 μL of acetone per animal) followed by TPA (12-o-tetradecanoyl-phorbol-13-acetate, 4 μg in 200 μL of acetone per animal), three times a week for 20 weeks starting at S0 experimental week and trichotomy every 15 days = G2 and G4; trichotomy every 15 days without induction of carcinogenesis = G1 and G3. Treatments: unloaded bacterial cellulose (G1 and G2); AgNMS (silver nimesulide complex) at 1 mg/1 cm2 in bacterial cellulose membrane (AgNMS@BCM) (G3 and G4); Parameters: Relative organ weight = ratio between organ weight (liver, kidney, testis or spleen) and the respective mice body weight; Hematological parameters: WBC = white blood cell; RCB = red blood cell; HGB = hemoglobin; HCT = hematocrit; MCV = mean corpuscular volume; MCH = mean corpuscular hemoglobin; MCHC = mean cellular hemoglobin concentration; PLT = platelets.