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. 2022 Jan 26;11(2):159. doi: 10.3390/pathogens11020159

Figure 2.

Figure 2

Effect of inflammasome modifications on LtxA-exposed THP-1 cells. PMA-stimulated THP-1 cells (wt, NLRP3− or ASC−) were exposed to different concentrations of A. actinomycetemcomitans LtxA for 2 h. Significant differences between knockout cells and wt cells are indicated (* p ≤ 0.05. *** p ≤ 0.001). (A) IL-1β release into cell culture supernatants of the exposed cells determined by quantitative ELISA (mean ± SD, n = 3). Deletion of the inflammasome genes NLRP3 and ASC significantly inhibited LtxA-induced IL-1β release with significantly more inhibition in ASC− cells at low LtxA concentrations. (B) Cell viability estimated by quantitative uptake of neutral red by the LtxA exposed cells (mean ± SD, n = 3). Deletion of the inflammasome genes NLRP3 and ASC significantly inhibited LtxA-induced cell lysis with no significant differences between the two modifications.