Table 2.
Differential expression for all genes altered in zebrafish following extended duration 4NP, TCC or TCS exposure starting at 4 h post-fertilization through 5 days post-fertilization and short-term duration starting at 4 dpf through 5 dpf. Significant absolute log2-fold changes (value ≥ 0.75 and adjusted p-value < 0.1) in bold. (ND = no difference in expression). Purple background indicates significant differential expression across all 3 chemicals, yellow across 4NP and TCS, green across 4NP and TCC, and blue across TCC and TCS.
| Exposure Concentrations (nM) 0–5 Days | Exposure Concentrations (nM) 4–5 Days | ||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Gene Name | 4-nonylphenol | Triclosan | Triclocarban | 4-nonylphenol | Triclosan | Triclocarban | |||||||||||||
| 0.1 | 10 | 1000 | 0.1 | 1 | 10 | 0.01 | 1 | 100 | 0.1 | 10 | 1000 | 0.1 | 1 | 10 | 0.01 | 1 | 100 | ||
| Cardiovascular | |||||||||||||||||||
| fbxo32 | F-box protein 32 | −1.2 | −0.8 | −0.2 | 0.4 | 0.2 | 1 | −0.01 | 0.5 | 1.4 | 0.6 | 0.2 | −0.5 | 1.1 | 0.3 | 0.1 | 1.7 | −1.1 | −0.2 |
| tfr1b | transferrin receptor 1b | 0.4 | 0.2 | 0.3 | 0.1 | −0.1 | 0.2 | −0.03 | −0.1 | −1.2 | 0.6 | 0.8 | −0.2 | 0.2 | 0.1 | −0.4 | −0.9 | ||
| myl10 | myosin, light chain 10, regulatory | −0.1 | 0.04 | −0.3 | −0 | 0.1 | −0 | −0.2 | 0.2 | −0.1 | 0.3 | 0.4 | 0.8 | 0.2 | 0.2 | −0.1 | −0.8 | −0.7 | 0.1 |
| mat2aa | methionine adenosyltransferase II, alpha a | −0.1 | 0.1 | 0.02 | −0 | −0 | 0.4 | −0.01 | −0.5 | −1 | −0.1 | 0.2 | 0.9 | −0.1 | 0.2 | 0.3 | −0.1 | 0.2 | 0.05 |
| hbae1.1 | hemoglobin, alpha embryonic 1.1 | −0.1 | 0.01 | −0.3 | −0.8 | −0.3 | −0.3 | 0.1 | −0.6 | −0.4 | −0.4 | −0.2 | 0.9 | −0.6 | 1.1 | 1 | −0.7 | −1.6 | −0.2 |
| tcp1 | t-complex 1 | 0.3 | −0.2 | −0.1 | −0.9 | −0.6 | −0.2 | 0.2 | −1.2 | −0.2 | −0.1 | −0.3 | 0.2 | −1 | 0.3 | 0.3 | −0.3 | −0.1 | −0.2 |
| hbbe1.2 | hemoglobin beta embryonic- 1.2 | 0.2 | 0.3 | 0.04 | ND | −0.3 | −0.4 | 0.04 | −0.1 | −0.3 | −0 | −0.2 | 0.03 | −0.1 | 1.1 | 0.4 | −0.3 | −0.8 | −0.8 |
| Neurological | |||||||||||||||||||
| reep3b | receptor accessory protein 3b | 0.5 | 0.7 | 0.2 | 0.6 | 0.03 | 0.2 | −0.4 | 0.2 | −0.2 | 0.5 | 0.8 | 0.2 | 0.8 | 0.1 | 0.01 | −0.3 | −1.4 | 0.1 |
| syngr3b | synaptogyrin 3b | −0.03 | 0.7 | 0.3 | 0.9 | 0.1 | 0.2 | −0.1 | 0.3 | −0.3 | 0.5 | 1 | 0.1 | 0.9 | 0.1 | −0.1 | 0.3 | −0.2 | −0.04 |
| agr2 | anterior gradient 2 | 0.9 | 0.4 | −0.2 | −0.5 | −0.4 | −0.3 | 0.2 | −0.7 | −0.8 | −0.1 | −0.05 | 0.4 | −0.3 | 0.4 | 0.05 | −0.7 | −2.5 | −0.1 |
| crygm2d18 | crystallin, gamma M2d18 | −0.2 | −1 | −0.1 | 0.2 | 0.1 | −0.8 | 0.03 | 0.04 | −0.1 | −0.3 | 0.3 | −0.1 | −0.6 | 0.6 | 0.1 | −0.2 | −1 | 0.8 |
| elmo2 | engulfment and cell motility 2 | −0.2 | 0.4 | −0.1 | 0.2 | −0 | 0 | −0.3 | −0 | −0.2 | 0.4 | 0.8 | −0.04 | 0.4 | 0.5 | −0.1 | 0.5 | 1.4 | 0.02 |
| arr3b | arrestin 3b, retinal (X- arrestin) | 0.1 | 0.4 | −0.02 | −0.1 | 0.2 | 0.1 | −0.3 | −0 | −2.6 | 0.2 | 0.8 | −0.2 | 0.1 | −0.1 | −0.2 | 0.04 | −0.4 | 0.2 |
| opn1sw2 | opsin 1 (cone pigments), short-wave-sensitive 2 | −0.1 | −0.01 | 0.2 | −0.2 | −0.3 | −0.8 | −0.1 | −0.1 | −0.3 | −0.3 | −0.4 | 0.2 | −0.4 | −0.4 | −0.3 | −0.8 | −1.3 | −0.1 |
| si:ch211- 153b23.5 | si:ch211-153b23.5 | 0.9 | 0.3 | −0.2 | −1 | −0.9 | −0.6 | 0.1 | −0.9 | 0.6 | 0.7 | 0.5 | 1 | −0.8 | 0.8 | 0.1 | −0.4 | 0.4 | 0.7 |
| mgst3a | microsomal glutathione S- transferase 3a | 0.3 | −0.1 | 0.1 | −0.9 | −0.9 | −0.3 | 0.2 | −1.1 | −0.5 | −0.5 | −0.5 | −0.1 | −1 | 0.4 | 0.3 | 0.02 | −0.5 | −0.4 |
| rlbp1b | retinaldehyde binding protein 1b | 0.1 | 0.1 | 0.4 | −0 | −0 | 0.2 | 0.2 | 0.1 | −0.8 | 0.2 | −0.1 | 0.1 | 0.4 | 1.5 | 0.5 | −1.1 | −0.5 | 0.04 |
| Metabolic Processes | |||||||||||||||||||
| cel.1 | carboxyl ester lipase, tandem duplicate 1 | −1.2 | −0.7 | −0.3 | 0.6 | 0.4 | 0.2 | −0.3 | 0.5 | 0.4 | 0.1 | −0.3 | −0.5 | 1 | 0.2 | −0.3 | 0.05 | −0.6 | −0.5 |
| amy2a | amylase alpha 2A | −0.9 | −0.7 | −0.1 | 0.8 | 0.4 | 0.2 | −0.1 | −0.2 | 0.5 | 0.1 | −0.2 | −0.8 | 0.8 | 0.4 | −0.2 | 0.4 | 0.4 | −0.6 |
| prss59.2 | serine protease 59, tandem duplicate 2 | −0.8 | −0.4 | 0.04 | 0.3 | −0.1 | 0.2 | −0.1 | −1 | 0.1 | −0 | −0.2 | −0.4 | 0.4 | 1.1 | 0.2 | 0.1 | −0.3 | −0.4 |
| ela2l | elastase 2 like | −0.7 | −0.3 | 0.06 | 0.3 | −0.5 | −0.6 | 0.2 | −0.2 | −0.1 | −0.5 | −0.9 | −0.4 | 0.5 | 0.4 | −0.9 | 0.1 | −0.8 | −0.3 |
| pip5k1cb | phosphatidylinositol-4- phosphate 5-kinase, type I, gamma b | 0.4 | 0.7 | 0.4 | 0.6 | 0.3 | 0 | −0.4 | 0 | −0.2 | 0.5 | 0.9 | 0.1 | 0.8 | −0.2 | −0.3 | 0.7 | −0.3 | 0.4 |
| fabp10a | fatty acid binding protein 10a, liver basic | −0.9 | −0.1 | −0.1 | 0.2 | 0.01 | 0.3 | −0.1 | 0.04 | −0.1 | −0.1 | −0.02 | −0.7 | 0.4 | 0.6 | 0.2 | 0.4 | −0.9 | −0.2 |
| zgc:92590 | zgc:92590 | −0.9 | −0.3 | −1.1 | −0 | −0 | 0 | −0.1 | −0.3 | −1.6 | −0.1 | 0.2 | −0.2 | 0.1 | 0.8 | 0.1 | −0.03 | −1.1 | −0.9 |
| si:ch211- 234p6.10 | si:ch211-234p6.10 | −0.8 | −0.03 | 0.4 | 0 | −0.2 | 0.2 | −0.04 | 0.1 | −0.3 | 0.4 | 0.1 | −0.6 | 0.3 | 0.6 | 0.2 | 0.9 | 0.3 | −0.4 |
| lpin1a | lipin 1 | −0.8 | −0.4 | −0.4 | 0.1 | −0 | 0.2 | −0.2 | 0.2 | 0.9 | 0.2 | −0 | −0.3 | 0.4 | 0.02 | −0.1 | 0.6 | 0.2 | −0.03 |
| fkbp9 | FKBP prolyl isomerase 9 | 0.8 | 0.6 | −0.02 | −0.1 | −0.3 | 0 | −0.4 | −1.7 | −2 | −0 | 1 | 0.8 | −0.2 | −0.2 | 0.04 | −0.1 | −0.6 | −0.1 |
| abcc2 | ATP-binding cassette, sub- family C (CFTR/MRP), member 2 | −0.4 | 0.1 | −0.1 | 0.4 | 1 | 1 | −0.1 | 0.4 | 1 | 0.3 | 0.2 | 0.2 | 0.7 | −0.2 | 0.6 | −0.1 | 0.2 | −0.1 |
| plin2 | perilipin 2 | 0.06 | −0.2 | 0.6 | −0 | 0.1 | 1.1 | −0.2 | −0.3 | 1.1 | 0.8 | 0.4 | 0.4 | 0.3 | 1 | 1.1 | 0.4 | −0.6 | −0.1 |
| fdps | farnesyl diphosphate synthase (farnesyl pyrophosphate synthetase, dimethylallyltranstransferas e, geranyltranstransferase) | 0.1 | 0.4 | 0.1 | −0.1 | −0.4 | 0.4 | 0.02 | −0.1 | −1.2 | 0.03 | 0.4 | 0.3 | 0.5 | 1 | 0.6 | 0.04 | 0.3 | 0.1 |
| Immune System | |||||||||||||||||||
| irg1l | immunoresponsive gene 1, like | 1.5 | 0.2 | 0.04 | −0.5 | −0.2 | −0.2 | 0.2 | −0.7 | 1.8 | 0.8 | 0.4 | 0.6 | −0.3 | 0.8 | 0.2 | −0.3 | 0.6 | 1.1 |
| hsp90b1 | heat shock protein 90, beta (grp94), member 1 | 1 | 0.2 | 0.3 | −0.3 | −0.1 | −0.2 | 0.4 | −0.9 | 0.2 | 0.1 | −0.1 | 0.4 | −0.4 | −0.4 | 0.2 | −0.3 | −0.9 | −0.1 |
| ctsl.1 | cathepsin L.1 | −0.4 | 0.2 | −0.4 | 0.7 | 0.3 | −0.1 | −0.1 | −0.2 | 0.7 | 0.6 | 0.5 | −1.3 | 0.8 | 0.5 | −0.5 | 1 | −0.1 | −0.4 |
| Extracellular Processes | |||||||||||||||||||
| si:dkey-14d8.6 | si:dkey-14d8.6 | −1.1 | −0.4 | 0.01 | 1 | 0.4 | 0 | −0.2 | −0.2 | 0.5 | 0.2 | −0.2 | −0.3 | 0.9 | 0.4 | −0.5 | 0.5 | −0.2 | −0.4 |
| pdk2b | pyruvate dehydrogenase kinase 2b | −1.1 | −0.5 | 0.004 | −0 | 0.2 | 0.9 | −0.1 | 0.3 | 1.2 | 0.5 | −0.2 | −0.5 | 0.3 | 0.4 | 0.3 | 0.8 | 0.5 | −0.6 |
| cpa1 | carboxypeptidase A1 (pancreatic) | −1.3 | −0.9 | −0.5 | 0.1 | 0.02 | 0.3 | −0.1 | −0.6 | −0.2 | −0.1 | −0.3 | −0.6 | 0.3 | 0.4 | 0.3 | 0.9 | −0.8 | −0.4 |
| Intracellular Processes | |||||||||||||||||||
| si:ch211- 153b23.4 | si:ch211-153b23.4 | 1.2 | −0.2 | −0.3 | −0.9 | −0.5 | −0.5 | 0.2 | −0.4 | 1.4 | 0.8 | 0.1 | 0.8 | −0.5 | 1.2 | 0.5 | −0.3 | 0.05 | 0.5 |
| mt-tRNA | tRNA on mitochondrial genome | −0.002 | −0.04 | −0.05 | −0.9 | −0.4 | −0.6 | 0.1 | 0.4 | 0.5 | −0.3 | −1.1 | −0.4 | −0.4 | 0.2 | 0.1 | −1 | −1.9 | −0.4 |
| prelid3b | PRELI domain containing 3 | 0.1 | 0.4 | 0.3 | 1 | 0.4 | 0.5 | −0.4 | 0.1 | −0.5 | 0.4 | 0.8 | 0.1 | 1.3 | 0.3 | −0.1 | 0.2 | −0.6 | 0.2 |
| hnrnpa0l | heterogeneous nuclear ribonucleoprotein A0, like | 0.4 | 0.2 | −0.03 | −0.8 | −0.5 | −0.3 | 0.001 | −1 | −0.8 | 0.2 | 0.5 | 1 | −0.8 | 0.5 | 0.1 | −0.1 | 0.1 | −0.02 |
| calcoco1b | calcium binding and coiled- coil domain 1b | −1 | −0.7 | −0.3 | 0.1 | 0.1 | 0.4 | 0.1 | 0.7 | 2.2 | 0.4 | −0.2 | −1.3 | 0.7 | 0.7 | 0.2 | 0.9 | 1.1 | −0.1 |
| mknk2b | MAPK interacting serine/threonine kinase 2b | −0.9 | −0.5 | −0.2 | 0 | 0.1 | 0.2 | 0.03 | −0.1 | 1.1 | −0 | −0.2 | −0.4 | 0.3 | 0.1 | −0 | 0.6 | −0.1 | 0.1 |
| trim63a | tripartite motif containing 63a | −0.8 | −0.6 | −0.2 | −0.3 | −0.1 | 0.1 | 0.2 | 0.3 | 0.9 | 0.04 | −0.3 | −0.6 | 0.1 | 0.2 | −0.2 | 0.5 | −1.6 | −0.2 |
| si:ch211- 207n23.2 | si:ch211-207n23.2 | 0.9 | 0.3 | 0.03 | −0.5 | −0.4 | −0.5 | 0.3 | −0.3 | 1.5 | 0.8 | 0.5 | 0.2 | −0.3 | 0.5 | 0.1 | 0.2 | 0.3 | 0.4 |
| si:ch211- 153b23.3 | si:ch211-153b23.3 | 1.2 | −0.02 | −0.1 | −0.1 | −0 | −0 | 0.2 | 0.3 | 1.9 | 0.3 | 0.3 | 0.5 | −0 | 0.7 | 0.3 | −0.2 | 0.8 | 1.3 |
| sult5a1 | sulfotransferase family 5A, member 1 | 1.7 | 0.7 | 0.1 | −0.1 | −0 | −0.1 | 0.1 | −0.2 | 2.2 | 0.4 | 0.3 | 0.2 | −0.1 | 1 | 0.1 | −0.3 | 0.9 | 0.7 |
| mt-atp8 | ATP synthase 8, mitochondrial | −0.5 | −0.4 | −0.7 | −0.4 | 0.2 | 0 | −0.2 | 0.8 | 0.1 | −0.7 | −1 | 0.2 | −0.3 | −0.4 | 0 | −0.5 | 0.2 | −0.1 |
| dgcr8 | DGCR8 microprocessor complex subunit | 0.1 | 0.4 | 0.02 | 0.2 | 0.2 | 0.3 | −0.2 | 0.3 | −0.8 | 0.3 | 0.8 | 0.1 | 0.1 | −0.02 | 0.1 | 0.1 | 0.6 | 0.1 |
| bub3 | BUB3 mitotic checkpoint protein | 0.2 | 0.3 | −0.04 | −0.2 | −0.1 | −0.1 | −0.1 | −1.3 | −1.1 | 0.3 | 0.8 | 0.7 | −0.1 | −0.1 | −0.1 | −0.3 | −1 | 0.5 |
| xpot | exportin, tRNA (nuclear export receptor for tRNAs) | −0.3 | 0.2 | −0.3 | 0.1 | −0 | −0.1 | −0.4 | −0.8 | −1.5 | 0.3 | 0.8 | 0.4 | −0 | −0.1 | −0.2 | 0 | 0.4 | 0.9 |
| si:ch211- 250g4.3 | si:ch211-250g4.3 | 0.1 | 0.3 | 0.3 | 0.7 | 0.4 | 0.7 | 0.1 | 0.8 | 1 | 0.3 | 0.8 | −0.05 | 0.8 | 0.5 | 0.3 | 0.8 | −0.2 | −0.02 |
| cebpd | CCAAT enhancer binding protein delta | −0.1 | 0.03 | 0.04 | −0.4 | 0.1 | 0.6 | 0.2 | −0.2 | 1.2 | 0.1 | −0.5 | −0.8 | 0 | 0.4 | 0.4 | 0.9 | 0.01 | −0.2 |
| hnrnpa0a | heterogeneous nuclear ribonucleoprotein A0a | 0.5 | 0.2 | −0.1 | −0.5 | −0.4 | −0.4 | −0.1 | −0.5 | −0.8 | 0.2 | 0.4 | 0.8 | −0.8 | 0 | 0.02 | −0.02 | −0.4 | −0.3 |
| fkbp5 | FKBP prolyl isomerase 5 | −0.4 | 0.1 | 0.1 | 0.4 | 0.7 | 1 | 0.2 | −0.1 | 0.2 | 0.01 | −0.5 | 0.5 | 0.5 | −0.4 | ND | 0.8 | −0.02 | −0.03 |
| ddx39ab | DEAD (Asp-Glu-Ala-Asp) box polypeptide 39Ab | 0.3 | −0.1 | −0.1 | −1 | −0.6 | −0.4 | 0.1 | −0.6 | −0.9 | −0.1 | −0.2 | 0.3 | −1.2 | 0.3 | 0.3 | −0.6 | −0.9 | −0.2 |
| tyrp1b | tyrosinase-related protein 1b | 0.4 | −0.02 | 0.02 | −1 | −0.8 | −0.4 | 0.2 | −0.2 | −1.5 | −0.3 | −0.4 | −0.1 | −0.7 | 0.4 | 0.2 | −1 | −0.4 | −0.2 |
| hnrnpa0b | heterogeneous nuclear ribonucleoprotein A0b | 0.5 | 0.2 | 0.3 | −0.8 | −0.5 | 0.2 | 0.2 | −0.9 | −1.3 | −0.2 | −0.01 | 0.2 | −0.6 | 0.3 | 0.4 | 0.1 | 0.6 | −0.1 |
| phtf2 | putative homeodomain transcription factor 2 | −0.4 | 0.2 | 0.2 | 0.5 | 0.2 | 0.2 | −0.2 | 0.3 | −0 | 0.3 | 0.3 | −0.1 | 0.9 | 0.2 | 0 | −0.2 | −2.4 | 0.2 |
| zmp:0000001081 | zmp:0000001081 | 0.2 | 0.1 | 0.3 | 0.7 | 0.3 | 0.6 | 0.2 | 0.2 | 1.4 | 0.2 | 0.7 | −0.3 | 0.9 | 0.5 | 0.2 | 0.8 | 0.2 | 0.2 |
| si:ch211- 113a14.18 | si:ch211-113a14.18 | −0.003 | −0.1 | 0.02 | 0.1 | 0.3 | 0.1 | 0.1 | 0.7 | 0.1 | −0.1 | −0.2 | −0.3 | −0.1 | −1.4 | −0.5 | 0.2 | 2.4 | −0.1 |
| zgc:113263 | zgc:113263 | −0.1 | −0.2 | 0.1 | 0.2 | 0.01 | 0 | −0.1 | 0.2 | 0.4 | 0.3 | 0.2 | 0.2 | 0.01 | −1.2 | −0.2 | 0.2 | 1.4 | 0.1 |
| si:ch211- 132b12.7 | si:ch211-132b12.7 | 0.1 | 0.4 | −0.1 | 0.2 | 0 | 0.7 | −0.3 | −0.2 | −2 | −0.2 | 0.5 | −0.1 | 0.9 | 1 | 0.5 | 0.1 | −0.03 | −0.1 |
| tm4sf21b | transmembrane 4 L six family member 21b | −0.02 | −0.4 | −0.2 | −0.1 | −0 | 0.2 | 0.3 | −1 | −0.4 | −0.5 | −0.5 | 0.1 | 0.1 | 1.3 | 0.6 | −0.3 | 0.2 | −0.6 |
| Xenobiotic Signaling | |||||||||||||||||||
| cyp3a65 | cytochrome P450, family 3,subfamily A, polypeptide 65 | −1 | −0.6 | −0.1 | 0.5 | 1 | 0.7 | 0.1 | 0.6 | 2 | 0 | −0.5 | −0.8 | 0.5 | 0.1 | 0.1 | −0.1 | −0.6 | −0.4 |
| ucp1 | uncoupling protein 1 | −0.9 | −0.4 | 0.1 | 0.5 | 0.1 | 0.4 | −0.1 | 0.3 | 1.4 | 0.1 | −0.2 | −0.5 | 0.7 | 0.9 | 0.1 | 0.7 | −0.1 | −0.5 |
| cyp2k18 | cytochrome P450, family 2, subfamily K, polypeptide18 | 0.3 | −0.03 | 0.2 | 0.2 | 2 | 2.4 | −0.1 | −0.1 | 1.9 | 0.2 | −0.2 | 0.6 | 0.1 | −0.2 | 0.3 | −0.2 | 2.4 | −0.5 |