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(A) PCA and (B) oPLS-DA score plot of the 20-lipid panel differentiating sera from injured and uninjured male animals with 2 orthogonal components. (C) PCA and (D) oPLS-DA score plot of the 19-lipid panel differentiating sera from injured and uninjured female animals with 2 orthogonal components. Both panels were created with 10 iterations of Venetian blinds cross-validation and 200 iterations of permutation testing. Both procedures supported a lack of evidence for overfitting.
