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Metabolic pathways impacted by sample preparation methods. Pathway analysis was performed between (A) trypsinized vs scraped samples, and (B) freeze–thaw vs. homogenizer bead samples using MetaboAnalyst. Both the mummichog and GSEA algorithms were performed to determine the enriched pathways. Pathways with an integrated p-value less than 0.05 are annotated. Pathways are numbered in order of increasing integrated p-value.
