Table 1.
Oligonucleotide sequence of primers for RT-PCR.
Gene | Forward Primer | Reverse Primer | T° Annealing |
---|---|---|---|
Fas-L | 5′-CAAGTCCAACTCAAGGTCCATGCC-3′ | 5′-AGAGAGAGCTCAGATACGTTTGAC-3′ | 58 °C |
Bim | 5′-AACCTTCTGATGTAAGTTCT-3′ | 5′-GTGATTGCCTTCAGGATTAC-3′ | 58 °C |
p27 | 5′-TGCGAGTGTCTAACGGGAG-3′ | 5′-GTTTGACGTCTTCTGAGGCC-3′ | 59 °C |
Bcl-2 | 5′-ATGTGTGTGGAGAGCGTCAA-3′ | 5′-AGAGACAGCCAGGAGAAATCA-3′ | 47 °C |
BDNF | 5′-GGCTGACACTTTTGAGCACGTC-3′ | 5′-CTCCAAAGGCACTTGACTGCTG-3′ | 52 °C |
IL-1β | 5′-TCATGGGATGATGATAACCTGCT-3′ | 5′-CCCATACTTTAGGAAGACACGATT-3′ | 50 °C |
IL-6 | 5′-CTGGTGACAACCACGGCCTTCCCT-3′ | 5′-ATGCTTAGGCATAACGCACTAGGT-3′ | 54 °C |
TNF-α | 5′-AGCCCACGTCGTAGCAAACCA-3′ | 5′-GCAGGGGCTCTTGACGGCAG-3′ | 53 °C |
Β-actin | 5′-CGGGATCCCCGCCCTAGGCACCAGGGT-3′ | 5′-GGAATTCGGCTGGGGTGTTGAAGGTCTCAAA-3′ | 60 °C |