Table 2.

Clinical score scheme.

Category	Description of Signs	Score
Posture and appearance	Normal posture, smooth fur	0
	Normal posture, ruffled fur	1
	Mildly hunched back, ruffled fur	2
	Severely hunched back, ruffled fur, lack of cleaning	3
Behavior and activity	Curious and alert	0
	Calm, mildly reduced spontaneous movement	1
	Apathy, moderately reduced spontaneous movement, mildly reduced induced movement	2
	Stupor, no spontaneous movement, severely reduced induced movement	3
Body weight	No change >5%	0
	Decrease of 5–15%	1
	Decrease of 15–25%	2
	Decrease of >25%	3

Mice were euthanized after reaching a score of 3 in one category or a score of 2 in all categories.