Fig. 1.

Immunofluorescence labeling of T cells (CD3) with region of interest (ROI) and distance map analysis. a Labeling of T cells with Cy5 (red) and nuclei with DAPI (blue). b Backward gating of CD3⁺ T cells (green) using ROI analysis. c Backward gating of CD3⁺ T cells (green) with superimposed distance map. Backward gating is always on the nuclei shade. The Euclidean distance map consists of six regional zones from 0–50 µm (dark red) to 250–350 µm (bright yellow) in 50 µm steps. Locations of mesh fibers are marked with asterisks, scale bar = 100 µm. Images of explant #3 (color figure online)