Table 4.
Spatial analysis of innate immune cells and the proportions of “conventional” cells participating in the foreign body reaction using Euclidean distance maps
| Parameter (n = 7 PP meshes) | Distance from mesh fibers | |||||
|---|---|---|---|---|---|---|
| Zone1 000–050 µm |
Zone2 050–100 µm |
Zone3 100–150 µm |
Zone4 150–200 µm |
Zone5 200–250 µm |
Zone6 250–350 µm |
|
| Cell density (cells/ mm2) | 4,418.2 (80.2) | 3,602.8 (98.6)a | 2,849.3 (95.2) | 2,386.1 (89.7) | 2,018.9 (91.8) | 1,696.1 (108.4)b |
| % CD11b+ | 30.2 (5.0) | 15.2 (3.9) | 11.4 (2.7) | 9.9 (2.0) | 8.1 (1.2) | 9.0 (1.6)b |
|
% CD11b+CD68−/CD11b+ “conventional” |
37.2 (3.9) | 63.9 (5.6)a | 67.5 (5.4) | 68.0 (6.3) | 66.2 (6.2) | 65.7 (4.8)b |
| % CD56+ | 11.3 (2.9) | 7.0 (1.6) | 7.4 (1.9) | 8.4 (2.4) | 8.2 (2.4) | 8.8 (2.5) |
|
% CD56+CD68−/CD56+ “conventional” |
18.1 (2.9) | 61.9 (9.5)a | 73.4 (8.0) | 75.1 (5.6) | 78.2 (7.5) | 83.3 (5.6)b |
| % CD68+ | 45.4 (1.5) | 20.1 (1.4)a | 14.0 (1.0) | 11.8 (0.8) | 10.4 (0.8) | 9.7 (0.8)b |
The cell density is defined as the mean number of cells per mm2. Percentages of single-positive (e.g., % CD56+) cells in relation to all cells and the proportions of “conventional” cells with respect to the total number of positive cells (e.g., % CD56+CD68−/CD56+). Hybrid cells with co-expression of CD68 were considered “disturbed”. The data are presented as mean (SE). Significant differences between zones according to Mann–Whitney U test: azone1 and zone2, bzone1 and zone6