Figure 6. Erythropoietin (EPO) responders are multipotent progenitors, not hematopoietic stem cells (HSCs).

Same protocol as in Figure 5. (a) Expression of published gene signatures of HSCs (dormant HSC [Cabezas-Wallscheid et al., 2017], molecular overlap [molO] HSC signature [Wilson et al., 2015]) and multipotent progenitors (MPPs) (MPP1 [Cabezas-Wallscheid et al., 2017]-2 [Pietras et al., 2015]) across the entire dataset (see Materials and methods). (b) Expression of the signatures from (a), across control, nonresponder, and EPO responder groups as defined in Figure 5c. Statistical comparisons made using a Kruskal–Wallis test with a Dunn’s multiple comparisons post-hoc test. (c) Expression of the molO HSC signature on the published reference map (Dahlin et al., 2018). (d) Nearest-neighbor mapping of control, EPO responder, and nonresponder cells onto the published reference map (Dahlin et al., 2018).