Fig. 2.

Intra-batch data consistency and reproducibility based on multiple injections of chicken serum extract using RHIMMS. The PCA score plots show negligible variation over the vast majority of injections. Injections 1–5 in positive ionization mode (n = 300; 8881 molecular features), and injections 1–7 and 218–230 in negative ionization mode (n = 230; 3422 molecular features) fall outside the 95% confidence region of the cluster. List of ten selected annotations in chicken serum extract and the deviation of their retention time (RT) and peak area across 200 consecutive injections (recommended batch size) in positive and negative ionization modes are tabulated. ¹Measured m/z, ²RSD relative standard deviation