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. 2022 Feb 28;18(3):16. doi: 10.1007/s11306-022-01871-1

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Comparison of extracted ion chromatograms (EICs) obtained from RHIMMS (3.5-min HILIC-Z-IM-qTOF; (A), 30-min ZIC-pHILIC-IM-qTOF (B) and 30-min ZIC-pHILIC-qTOF (C), analyses in positive and negative ionization modes. EICs show selected metabolites from chicken serum extract, putatively identified as (1) Palmitoleic acid (16:1), (2) Nicotinate, (3) Betaine, (4) O-Acetylcarnitine, (5) l-Carnitine, (6) Arachidonic acid, (7) Thymidine, (8) l-Phenylalanine, (9) l-Tyrosine, (10) Glutamine. EICs are scaled to the largest in each chromatogram. Measured m/z, retention time (RT), drift time (tD) and CCS values for annotated metabolites, as obtained from each method are tabulated for comparison. ¹Experimentally derived CCS values (DB) from Unified CCS Compendium