Figure 2.
Effect of UVA irradiation on inflammatory mediators release in SZ95 sebocytes
(A) The mRNA expression levels of IL-1α, IL-1β, IL-6, IL-8, and protein quantitation by ELISA of IL-6 and IL-8 in SZ95 sebocytes after 24 and 48h post 1-UVA and 48h post three or 4-UVA 5 J/cm2 irradiations. For mRNA levels, results are expressed as the fold change respect to untreated control cells (∗p < 0.05, ∗∗p < 0.01). For ELISA assay, results are expressed in absolute quantities (∗p < 0.05, ∗∗p < 0.01 vs Ctr).
(B) PGD2, PGE2, PGF2α, LTB4, and AA quantitation by HPLC-MS/MS in SZ95 sebocytes after 24 and 48h post 1-UVA and 48h post three or 4-UVA 5 J/cm2 irradiations. Results are expressed in absolute quantities (∗p < 0.05, ∗∗p < 0.01 vs Ctr). Data represent the mean ± SD of three independent experiments.